论文部分内容阅读
为分析2009年~2015年河南省5岁以下腹泻儿童A组、B组、C组轮状病毒的感染状况及分子流行病学特征,本研究采集了河南省两个监测哨点医院5岁以下儿童腹泻病例的粪便样本(每份3~5ml)2 317份,双抗体夹心法检测A组轮状病毒;巢式多重RT-PCR进行基因分型,二步法多重RT-PCR检测B/C组轮状病毒,同时对阳性病例流行病学资料进行统计分析。2 317份腹泻样本共检出阳性样本869份,总阳性率35.0%,混合感染率2.5%。其中A组轮状病毒746份,阳性率32.2%;B组轮状病毒44份,阳性率1.9%;C组轮状病毒79份,阳性率3.4%;混合感染样本58份,混合感染率2.5%。A组轮状病毒阳性率的季节性特征显著,监测年度存在9~11月份(秋季)和3~5月份(春季)两个较显著高峰。A组轮状病毒G分型以G1、G2、G3、G9为主;P分型以P[8]、P[4]为主;型别组合以G1P[8]、G3P[8]、G2P[4]、G9P[8]为主,还存在G13P[8]、G234P[8]、G39P[8]、G389P[6][8]混合感染型别。河南省5岁以下腹泻患儿中以A组轮状病毒感染为主,B/C组轮状病毒感染率较低,且存在与A组混合感染模式;秋季和春季为高发季节,秋季高于春季且存在混合感染病例;大部分感染病例无发热、呕吐、脱水症状,出现发热、腹泻、呕吐、脱水等临床症状的病例多以轻症为主。
In order to analyze the infectious status and molecular epidemiological characteristics of rotavirus in group A, B and C of children under 5 years old in Henan province from 2009 to 2015, two surveillance sentinel hospitals in Henan Province were collected under the age of 5 A total of 2 317 stool samples from children with diarrhea (3-5ml each) were collected. A group of rotavirus was detected by sandwich enzyme-linked immunosorbent assay (NACR). Nested multiplex RT-PCR was used for genotyping. Two-step multiplex RT-PCR was used to detect B / C Group rotavirus, while the positive cases of epidemiological data for statistical analysis. A total of 869 positive samples were detected in 2 317 diarrhea samples, with a total positive rate of 35.0% and a mixed infection rate of 2.5%. Among them, 746 cases of group A rotavirus had a positive rate of 32.2%; 44 cases of group B rotavirus had a positive rate of 1.9%; 79 cases of group C rotavirus with a positive rate of 3.4%; 58 cases of mixed infection and a mixed infection rate of 2.5 %. A group of rotavirus positive rate of the seasonal characteristics of significant, monitoring the existence of 9 to November (autumn) and March to May (spring) two significant peaks. Group A was mainly G1, G2, G3 and G9; P was mainly P [8] and P [4]; Type combinations were G1P [8], G3P [8], G2P [4], G9P [8], mixed infection types of G13P [8], G234P [8], G39P [8] and G389P [6] [8]. The majority of children with diarrhea under 5 years of age in Henan Province were infected with group A rotavirus, and the infection rate of rotavirus was low in group B / C and there was a mixed infection pattern with group A. Diarrhea and spring were the high incidence seasons, In spring, there are mixed infections; most cases of infection have no symptoms of fever, vomiting, dehydration, fever, diarrhea, vomiting, dehydration and other clinical symptoms.