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目的制备生物素化脂质微泡,并应用平行板流动腔检测流体切应力对生物素化脂质微泡与链亲和素结合稳定性的影响,为进一步开展超声分子成像研究奠定基础。方法采用声振法制备出表面携有生物素分子的脂质微泡,与普通脂质微泡对照,应用平行板流动腔模型,设置不同流体剪切应力,观察与链亲和素靶向结合的生物素化微泡的黏附效果。结果在不同浓度链亲和素包被的平行板流动腔中均见生物素化微泡结合;随着链亲和素包被浓度的提高,靶向结合的生物素化脂质微泡抗流体剪切应力能力明显提高。结论应用平行板流动腔模型能成功检测生物素化微泡的靶向黏附效果,该模型可推广应用于其他靶向微泡制备成功后靶向黏附能力的体外检测。
OBJECTIVE: To prepare biotinylated lipid microbubbles and to investigate the effect of fluid shear stress on the binding stability of biotinylated lipid microbubbles to streptavidin by using a parallel plate flow cell, which lays the foundation for further research on molecular imaging of ultrasound. Methods Lipid microbubbles with biotin molecules on the surface were prepared by acoustic vibration method. Compared with ordinary lipid microbubbles, the parallel plate flow cavity model was used to set the shear stress of different fluids. The targeted binding with streptavidin Of the biotinylated microbubbles adhesion effect. Results Biotinylated microbubble binding was observed in parallel-flow plates coated with streptavidin at different concentrations. As the streptavidin-coated concentration was increased, the targeted bound biotinylated lipid microbubbles anti-fluid Shear stress ability improved significantly. Conclusion The parallel plate flow cavity model can successfully detect the targeted adhesion of biotinylated microbubbles. This model can be applied to the in vitro detection of target adhesion ability after the successful preparation of other targeted microbubbles.