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为了研究剧烈运动结束后心肌组织损害的变化规律，本文对无训练的ＳＤ雄性成年大鼠（体重２５０－３００ｇ）随机分为对照组（不运动）、运动后即刻组、运动后３小时组、运动结束后ｚ４小时组和运动结束后１００小时组。所有运动组大鼠均以２０－２５米／分，持续在啮齿类动物跑台运动４００－４５００米。各组动物处死后，取心肌腱索乳头肌制冰冻超薄切片，在电子显微镜下以Ｘ线能谱分析测定大鼠心肌线粒体内钙含量，并测定心肌组织匀浆内酸性磷酸酶（ＡＣＰ）和β葡萄糖醛酸酶（Ｂｅｔａ－ｇｌｕｃｕｒｏｎｉｄａｓｅ）的动态变化．结果提示，“肌线粒体内钙在运动后即刻显著性增高，运动２４小时组心肌线粒体内钙进－步增力’：运动后即刻心肌ＡＣＰ和β－葡萄糖醛酸酶明显增加，运动结束后有延迟性增加现象。
In order to study the changes of myocardial tissue damage after strenuous exercise, SD adult male rats (weight 250-300g) were randomly divided into control group (no exercise), immediate exercise group, 3 hours after exercise group, Z4 hours after exercise and 100 hours after exercise. All sports rats were 20-25 m / min, continued in rodents treadmill 400-4500 meters. After the animals were sacrificed, the myocardium chondrocytes were cryostatically sectioned and the mitochondrial calcium content was determined by X-ray spectroscopy under an electron microscope. The contents of acid phosphatase (ACP) And β-glucuronidase (Beta-glucuronidase) dynamic changes. The results suggest that "myocardial mitochondrial calcium immediately after exercise increased significantly, exercise 24 hours of myocardial mitochondrial calcium in the progressive step-force ’: immediately after exercise myocardial ACP and β-glucuronidase increased significantly after exercise Delayed increase phenomenon.