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以2个金铁锁四倍体材料(T1、T2)及1个二倍体金铁锁组培苗(CK)为试材,采用石蜡切片法,观察不同倍性金铁锁组培苗的茎、叶解剖结构差异。结果表明:不同倍性金铁锁组培苗叶及茎的相关性状均差异显著。不同倍性金铁锁均无明显栅栏组织和海绵组织的分化。四倍体金铁锁T1与T2叶片厚度分别是二倍体的1.77、1.60倍;叶主脉厚度分别是二倍体的2.34、1.67倍。四倍体金铁锁T2的茎直径横切大小为二倍体金铁锁的1.40倍,而T1和T2的茎导管横切大小分别是二倍体的1.81、1.23倍;四倍体T1和T2的茎中空横切大小分别是二倍体的1.80、1.73倍。可见,金铁锁经多倍化处理后,其茎、叶的解剖结构发生了显著变异。研究结果可为金铁锁多倍体育种提供参考依据。
The anatomical structure of the stem and leaves of the tissue culture seedlings of different ploidy plants were observed by using paraffin section method with two tetraploid materials (T1 and T2) and one diploid (Camellia sinensis) tissue culture seedling (CK) . The results showed that there were significant differences in the traits of leaves and stems between different ploidy groups. Different ploidy lock did not show palisade tissue and sponge tissue differentiation. The thickness of T1 and T2 leaves of tetraploid metal lock were 1.77 and 1.60 times that of diploid and 2.34 and 1.67 times of diploid respectively. The diameter of stem diameter of tetraploid metal lock T2 was 1.40 times that of diploid iron lock, while the cross-sectional size of stem conduit of T1 and T2 were 1.81 and 1.23 times that of diploid, respectively. The stem hollows of tetraploid T1 and T2 The cross-sectional size is diploid 1.80, 1.73 times respectively. Can be seen, after the multi-fold gold lock treatment, the stem and leaf anatomical structure of a significant mutation occurred. The results can provide a reference for polyploid breeding.