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以甜瓜(Cucumis melo L.)感白粉病品种‘Hami413’为受体亲本,抗白粉病品种‘PMR6’为供体亲本构建的255株BC2分离群体为材料,研究‘PMR6’抗白粉病的遗传规律及基因定位。群体遗传分析表明,BC2群体中对白粉病菌Podosphaera xanthii生理小种1的抗性由显性单基因控制,基因命名为Pm-PMR6-1;利用混合分组分析法(Bulked segregant analysis,BSA)从分布于甜瓜12个连锁群上的390个SSR分子标记中筛选出5个多态性标记;通过连锁分析,将该抗性基因定位于12号连锁群SSR标记DM0191与CMBR111之间;根据甜瓜基因组信息设计SSR引物,进一步将该基因定位于SSR12407与SSR12202之间,并且该抗性基因与标记Mu7191共分离;比对基因组序列,两标记间物理距离约226 kb,预测35个候选基因。
A total of 255 BC2 segregating populations constructed from a susceptible cultivar ’Hami413’ of melon (Cucumis melo L.) were used as parents and resistant cultivars ’PMR6’ were used as materials to study the genetic relationship of ’PMR6’ resistant to powdery mildew Law and gene mapping. Population genetic analysis indicated that the resistance to the powdery mildew pathogen Podosphaera xanthii 1 in BC2 population was controlled by a dominant single gene and the gene was named Pm-PMR6-1. Bulked segregant analysis (BSA) Five polymorphic markers were screened from 390 SSR markers in 12 linkage groups of melon. The linkage analysis indicated that the resistance gene was located between SSR markers DM0191 and CMBR111 in linkage group 12. According to the melon genome information The SSR primer was designed to further locate the gene between SSR12407 and SSR12202, and the resistance gene was co-segregated with the marker Mu7191. The genome sequence was aligned with a distance of about 226 kb and the predicted 35 candidate genes.