腺病毒载体 (AdV)是一种重要的基因转导系统。腺病毒依两种不同的受体侵入靶细胞 :Ad纤维蛋白与CAR作用 ,介导病毒黏附细胞 ;Ad五邻体基底蛋白与整合素αvβ3 及αvβ5结合 ,促进病毒内化。靶细胞的CAR和整合素αvβ3 及αvβ5表达水平与AdV介导的基因转导效率直接相关 ,受体低表达限制AdV的转导率。动力蛋白调节Ad进入靶细胞 ,整合素聚集与肌动蛋白相关。Ad侵入靶细胞需要的信号通路包括GTP酶Pho家族成员P13K和肌动蛋白单纤维聚合。 Adenovirus vector (AdV) is an important gene transduction system. The adenovirus invades target cells at two different receptors: Ad fibrin and CAR act to mediate adhesion of virus; Ad pentamer base protein binds to integrins αvβ3 and αvβ5, promoting internalization of the virus. Target cells CAR and integrin αvβ3 and αvβ5 expression levels are directly related to AdV-mediated gene transduction efficiency, and receptor low expression limits AdV transduction. The dynein regulates Ad entry into target cells, integrin aggregation associated with actin. The signaling pathways required for invasion of target cells by Ad include P13K, a member of the Pho family of GTPases, and actin monofilament polymerization.