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研究了高效毛细管区带电泳分离人血清蛋白质的电泳行为及实验条件 ,建立了分离血清蛋白质的高效毛细管区带电泳法。血清样品经硼酸缓冲液 (5 0 mmol/L,p H8.80 )稀释后 ,以 0 .1 mol/L硼酸缓冲液 (p H9.3 5 ,含4g/L PEG80 0 0 )为电泳介质 ,在 5 0 μm i.d.× 3 7cm弹性石英毛细管柱 (有效柱长为 3 2 cm)中进行电泳分离 ,以2 0 0 nm紫外波长检测。方法简便、快速、重复性好 ,1 2 min内便可完成对血清中蛋白质的电泳分离。
The electrophoresis behavior and experimental conditions of high performance capillary zone electrophoresis separation of human serum proteins were studied. A high performance capillary zone electrophoresis method was established for the separation of serum proteins. Serum samples were diluted with boric acid buffer (50 mmol / L, p H 8.80) and the buffer solution (p H9.3 5, 4 g / L PEG80 0 0) was used as the electrophoresis medium in 0.1 mol / Electrophoretic separation was performed on a 5 0 μm id × 3 7 cm elastic silica capillary column (effective column length: 32 cm) and detected at 200 nm UV wavelength. The method is simple, rapid and reproducible, and the separation of proteins in serum can be completed within 12 minutes.