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小鼠为疟疾实验研究最常用的动物。寻求出小鼠对约氏疟原虫易感的品系(株),对疟疾实验研究是非常需要的。本文报告9株近交小鼠及一株远交小鼠对约氏疟原虫(Plasmodium yoelii yoclii)—斯氏按蚊(Anopheles stephensi)系统的敏感性的实验结果,经蚊传和血传试验表明:(1)输血转种所有小鼠均极敏感,而子孢子感染则各鼠株之间有差异,SMMC/C株等6个鼠株最为敏感,阳性率为100％;(2)子孢子感染的原虫血症出现时间,以SMMC/C株最早,並且出现时间比较一致;(3)斯氏按蚊对各阳性鼠株的易感性以SMMC/C株的腺感染率最高(73.20±59.2％)。因而,认为小鼠SMMC/C株—斯氏按蚊系统作为鼠疟实验动物模型较为适宜。另外,本文还报告,在约氏疟原虫感染SMMC/C鼠株实验中发现(1)每鼠接种子孢子5000个可使全部鼠感染上;(2)子孢子在鼠血清葡萄糖生理盐水在3～5 C或23～25 C下体外保存24小时后,90％以上的小鼠被感染上;(3)环境温度18～20 C对小鼠感染疟原虫发育最适宜;(4)幼鼠对子孢子感染最敏感,成年鼠次之。 对本试验结果进行了讨论。
Mice are the most commonly used animal for malaria research. The search for a mouse strain that is susceptible to Plasmodium yoelii (strain) is highly desirable for malaria research. In this paper, we report the experimental results on the sensitivity of 9 inbred mice and one outbred mouse to Plasmodium yoelii yoclii-Anopheles stephensi system. The results of mosquito and blood tests : (1) All the mice transplanted with transfusion were extremely sensitive, while the sporozoites were infected with different strains. The 6 strains of SMMC / C were the most sensitive, the positive rate was 100%. (2) The sporozoites (3) Susceptibility of An. Anopheles to each positive mouse strain The highest infection rate of gonads was found in SMMC / C strain (73.20 ± 59.2 %). Therefore, it is considered that mouse SMMC / C strain-An. Anopheles stephensi system is suitable as an experimental animal model of murine malaria. In addition, this paper also reported in experiments on Plasmodium yoelii infected SMMC / C mice (1) inoculated 5000 spores per mouse infected all mice; (2) sporozoites in rat serum glucose saline at 3 More than 90% of the mice were infected after being preserved in vitro at ~ 5 C or 23 ~ 25 C for 24 hours. (3) The optimal temperature for the development of malaria parasites in mice was 18 ~ 20 C at ambient temperature. (4) Sporozoite infection is the most sensitive, followed by adult mice. The test results were discussed.