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目的分析社区获得性肺炎(CAP)患儿Epstein-Barr病毒(EBV)及肺炎支原体(MP)感染的临床特点。方法选择2015年6月至2016年6月在齐齐哈尔医学院附属第三医院住院治疗的CAP患儿280例,采血,分离血清,采用酶联免疫吸附法(ELISA)检测EBV抗体,采用被动颗粒凝集法检测MP抗体,采用实时荧光定量PCR(qPCR)检测EBV-DNA、MP-DNA。结果 ELISA和被动颗粒凝集试验检测280例CAP患儿单一EBV、MP感染率及EBV+MP混合感染率分别为7.9%(22/280)、31.1%(87/280)和18.9%(53/280),以单一MP感染占多数(P<0.05);不同性别患儿单一EBV、MP感染率及EBV+MP混合感染率差异无统计学意义(P>0.05);按年龄统计单一EBV感染率及EBV+MP混合感染率以3月~1岁组较高(P<0.05),单一MP感染率>5岁~12岁组较高(P<0.05)。随病程的延长,患儿血清EBV-DNA阳性率逐渐降低,MP-DNA阳性率先升高后降低,并于入院后1周达最高(48.7%)。结论 CAP患儿以单一MP感染多见,EBV感染常与MP感染同时存在,且不同年龄患儿EBV、MP感染率不同,动态监测血清EBV-DNA、MP-DNA有助于儿童CAP的早期诊断及疾病进展评估。
Objective To analyze the clinical features of Epstein-Barr virus (EBV) and Mycoplasma pneumoniae (MP) infection in children with community-acquired pneumonia (CAP). Methods Seventy two children with CAP admitted to the Third Affiliated Hospital of Qiqihar Medical College from June 2015 to June 2016 were enrolled in this study. Serum was collected and serum was collected. EBV antibody was detected by enzyme-linked immunosorbent assay (ELISA) MP antibody was detected by real-time fluorescence quantitative PCR (qPCR) to detect EBV-DNA and MP-DNA. Results The single EBV infection rate was found in 280 cases of CAP with ELISA and passive particle agglutination test. The infection rates of MP and EBV + MP were 7.9% (22/280), 31.1% (87/280) and 18.9% (53/280), respectively ), And single MP infection accounted for the majority (P <0.05). There was no significant difference in single EBV, MP infection rate and EBV + MP mixed infection rate among different sexes (P> 0.05) The mixed infection rate of EBV + MP was higher in the 3 months to 1 year old group (P <0.05), and the infection rate of single MP> 5 years old to 12 years old group was higher (P <0.05). With the prolongation of the disease, the positive rate of serum EBV-DNA in children gradually decreased, and the positive rate of MP-DNA increased firstly and then decreased, and reached the highest one week after admission (48.7%). Conclusions Single MP infection is common in CAP children. EBV infection often coincides with MP infection. EBV and MP infection rates are different in different age groups. Dynamic monitoring of serum EBV-DNA and MP-DNA may be helpful for the early diagnosis of CAP in children And disease progression assessment.