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目的:研究单纯疱疹病毒2型（HSV-2）病毒抗原抗体阳性率及载量与T淋巴细胞亚群的相关性。方法:收集2017年4月至2019年11月我院收治的生殖器疱疹患者52例，另选择同期来院常规体检者30例作为对照组。按照生殖器疱疹发作次数将患者分为初发组24例与复发组28例。抽取各组静脉血，采用流式细胞仪检测T淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+表达水平。采用酶联免疫吸附试验检测HSV-2抗原及HSV-2型IgG和IgM阳性率。采用荧光定量PCR技术检测初发及复发组生殖器拭子中HSV-2-DNA载量。采用线性相关分析HSV-2-DNA载量与T淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+表达水平的相关性。结果:初发组及复发组T淋巴细胞亚群CD3+、CD4+、CD8+百分比及绝对值、CD4+/CD8+绝对值均低于对照组（n P<0.05）；复发组T淋巴细胞亚群CD3+、CD4+、CD8+百分比及绝对值、CD4+/CD8+绝对值均低于初发组（n P<0.05）；初发组HSV-2抗原及HSV-2型IgM阳性率高于复发组（n P<0.05）；初发组HSV-2型IgG阳性率低于复发组（n P<0.05）。复发组HSV-2-DNA载量高于初发组（n P<0.05）。初发组与复发组中T淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+均与HSV-2-DNA载量呈负相关（n P<0.05）。n 结论:生殖器疱疹初发患者HSV-2抗原及HSV-2型IgM阳性率较高，IgG阳性率较低，HSV-2-DNA载量与患者外周血T淋巴细胞亚群成反比，通过检测T淋巴细胞亚群数量，可能可对生殖器疱疹复发几率进行评估。“,”Objective:To study the correlation of the positive rates of the antigen, antibodies as well as the viral load of herpes simplex virus type 2 (HSV-2) with T lymphocyte subsets.Methods:A total of 52 patients with genital herpes in our hospital from April 2017 to November 2019 were enrolled. Thirty individuals for physical examinations in our hospital at the same period of time were selected as the control group. Patients were divided into initial group of 24 patients and the recurrent group of 28 patients according to the attack times. Venous blood was collected in each group, and the expression levels of CD3n +, CD4n +, CD8n + and CD4n +/CD8n + in T lymphocyte subsets were detected by flow cytometry. The positive rates of HSV-2 antigen as well as IgG and IgM against HSV-2 were determined by enzyme linked immunosorbant assay (ELISA). Fluorescence quantitative PCR was used to detect HSV-2-DNA loads in the initial and recurrent groups. The correlation of HSV-2-DNA load and expression levels of CD3n +, CD4n +, CD8n + and CD4n +/CD8n + in T lymphocyte subsets were calculated by linear correlation analysis.n Results:The percentages and absolute values of CD3n +, CD4n + and CD8n + and the absolute values of CD4n +/CD8n + of control group were higher than those of initial group (n P<0.05). The percentages and absolute values of CD3n +, CD4n + and CD8n + and the absolute values of CD4n +/CD8n + of initial group were higher than those of recurrent group (n P<0.05). The positive rate of HSV-2 antigen and IgM against HSV-2 of initial group was higher than that of recurrent group (n P<0.05). The positive rate of IgG against HSV-2 in initial group was lower than that in recurrent group (n P<0.05). HSV-2-DNA load in recurrent group was higher than that in initial group (n P<0.05). The expression levels of CD3n +, CD4n +, CD8n + and CD4n +/CD8n + in T lymphocyte subsets in initial group and recurrent group were all negatively correlated with HSV-2-DNA load (n P<0.05).n Conclusions:The initial patients of genital herpes showed high positive rates of HSV-2 antigen and IgM against HSV-2, as well as low positive rate of IgG. HSV-2 viral load was negatively proportional to the T lymphocyte subsets in peripheral blood of the patient. Quantitative detection of T lymphocyte subsets would be useful in assessment of genital herpes recurrence.