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从680名疑有自身免疫性疾病的非糖尿病受检者血清中筛检了13种不同的抗体,其中582人血清中发现含有这些抗体,九种抗体在一种IgG特定酶结合免疫吸附试验中凝集胰岛素,[微型酶免疫吸附试验(micro ELISA)],四种血清凝集人、猪和牛的胰岛素,五种只能和人的胰岛素起反应。“病的”人、猪和牛的胰岛素可置换(在剂量上按方法而定),所有三种胰岛素凝集四种血清,唯独人的胰岛素置换剩余的五种,在浓度达到1000u/L时,猪和牛的胰岛素几乎很少或没有作用。这些观察指出,在某些确有自身免疫性的人体中存在着抗体胰岛素的特异性的自身抗体。
Thirteen different antibodies were screened from sera of 680 non-diabetic subjects suspected of having autoimmune disease, of which 582 were found to contain these antibodies in serum, and nine of the antibodies were tested in an IgG-specific enzyme-linked immunosorbent assay Agglutination of insulin, [micro ELISA], four serums agglutinate human, porcine and bovine insulin, five of which react only to human insulin. The “sick”, pigs and bovine insulin are displaceable (in a dose-wise manner), all three insulin agglutinates in the four sera, except human insulin replacement of the remaining five, at concentrations up to 1000 u / L, Pigs and cattle have little or no insulin. These observations point to the existence of autoantibodies specific for antibody insulin in certain autoimmune humans.