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本文主要是观察促乳素(PRL)是否影响体外培养的大鼠颗粒细胞中,组织纤溶酶原激活因子(tPA)和1型纤溶酶原激活因子抑制因子(PAⅠ-Ⅰ)基因表达间的协调作用。我们采用了多种方法,例如SDS-PAGE、免疫印迹等,来检测PRL对tPA和PAI-I基因表达的作用。结果证实:(1)在离体条件下促乳素(PRL)能刺激颗粒细胞(GC)中PAⅠ-ⅠmRNA的合成,而FSH无此作用。但FSH可与PRL协同增加GC中PAⅠ-ⅠmRNA的产生。培养到48h,PRL和FSH协同增加GC中PAⅠ-ⅠmRNA含量是PRL单独作用的7.8倍,并且显示出明显的剂量和时间依赖关系;(2)PRL或PRL加FSH对GC中PAⅠ-ⅠmRNA合成的刺激作用,与细胞分泌到培液中的PAⅠ-Ⅰ活性的增加完全一致;(3)与此相反,PRL能显著抑制FSH所诱发的GC中(tPA)mRNA和蛋白的合成,并且也有剂量依赖关系。同时发现PRL对FSH诱发的tPA活性的抑制作用与培液中PA-PAⅠ-Ⅰ复合物形成量的增加相符,这说明tPA活性的下降与PAⅠ.Ⅰ对其的中和作用有关。
This article is mainly to observe whether prolactin (PRL) affects the expression of tissue plasminogen activator (tPA) and type I plasminogen activator inhibitor (PAⅠ-Ⅰ) in rat granulosa cells cultured in vitro The coordinating role. We used a variety of methods, such as SDS-PAGE, Western blot, etc., to detect PRL on tPA and PAI-I gene expression. The results showed that: (1) prolactin (PRL) stimulated the synthesis of PAI-I mRNA in granulosa cells (GC) in vitro without FSH. However, FSH synergizes with PRL to increase the production of PAI-I mRNA in GC. After cultured for 48h, PRL and FSH synergistically increased the content of PAⅠ-Ⅰ mRNA in GC, which was 7.8-fold higher than that of PRL alone, and showed a dose-dependent and time-dependent manner. (2) PAI-ⅠmRNA (3) In contrast, PRL significantly inhibited the synthesis of (tPA) mRNA and protein in GC induced by FSH, and there was also a significant increase in PAI-I activity Dose-dependent relationship. At the same time, it was found that the inhibitory effect of PRL on FSH-induced tPA activity was consistent with the increase of PA-PAI-I complex formation in the culture medium, indicating a decrease in tPA activity and PAI. Ⅰ on its role in the neutralization.