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目的观察我国黑龙江、河南及云南省3个地理株旋毛虫对小鼠的感染性。方法取30只昆明小鼠,随机分为3个组,用灌胃感染法分别建立黑龙江株、河南株和云南株旋毛虫动物感染模型,每只灌胃200条肌幼虫。取小鼠膈肌压片,镜下观察各株旋毛虫是否成囊,摸索成囊时间,同时计数膈肌虫荷;随机测量30个囊包的长和宽,计算囊包指数(长/宽);收集小鼠全身各部位肌肉中的幼虫,计算旋毛虫的繁殖力指数(RCI)。结果黑龙江株、河南株及云南株旋毛虫在昆明小鼠体内形成囊包的时间分别为53、40和40d;50mg小鼠膈肌虫荷数分别为(91.8±5.119)条/50mg、(301.8±14.839)条/50mg和(176.4±7.127)条/50mg;囊包指数分别为2.360±0.624、2.385±0.335和1.883±0.401,差异有统计学意义(P<0.05);RCI分别为66.20±1.569、293.84±3.855和154.80±3.894,差异有统计学意义(P<0.05)。结论河南株旋毛虫对小鼠的感染性较黑龙江株和云南株强。
Objective To observe the infectivity of Trichinella spiralis in three geographic strains of Heilongjiang, Henan and Yunnan in China. Methods Thirty Kunming mice were randomly divided into three groups. Animal models of Trichina infection in Heilongjiang, Henan and Yunnan strains were established by intragastric inoculation, and 200 muscle larvae were perfused. The mice were randomly divided into three groups. Diaphragmatic muscle strips of mice were used to observe whether Trichinella spiralis was encapsulated or not, and the time of capsule formation was explored. The length and width of 30 capsules were measured randomly. The larvae in the muscles of various parts of the mouse body were collected and the Trichinella infertility index (RCI) was calculated. Results The time of encapsulation of Heilongjiang strain, Henan strain and Yunnan strain Trichinella spiralis in Kunming mice were 53, 40 and 40 days, respectively. The numbers of diaphragm worms in 50mg mice were 91.8 ± 5.119 / 14.839) / 50mg and (176.4 ± 7.127) / 50mg, respectively. The values of encapsulation index were 2.360 ± 0.624, 2.385 ± 0.335 and 1.883 ± 0.401 respectively, with statistical significance (P <0.05). RCI were 66.20 ± 1.569, 293.84 ± 3.855 and 154.80 ± 3.894, respectively. The difference was statistically significant (P <0.05). Conclusion The infection of Trichinella spiralis strains in mice is stronger than that in Heilongjiang strain and Yunnan strain.