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目的:探讨转录因子Slug基因沉默对前列腺癌细胞LNCaP缺氧后上皮间质转化(Epithelial-mes-enchymal transition,EMT)过程的影响及其相关的分子机理。方法:利用shRNA转染技术建立Slug沉默LNCaP细胞系,通过缺氧诱导EMT。采用western blot检测EMT发生的标志分子E-cadherin和N-cadherin蛋白的表达水平变化。Transwell侵袭实验评价细胞侵袭能力的改变。结果:成功建立Slug-shRNA稳定转染细胞系;缺氧诱导后,对照组E-cadherin表达水平明显降低,而N-cadherin表达水平明显升高,但是,在Slug沉默组,二者表达无明显变化;Slug沉默抑制了缺氧诱导的细胞侵袭能力的增加。结论:Slug基因沉默能够抑制缺氧诱导前列腺癌细胞EMT的发生;Slug与前列腺癌转移密切相关。
Objective: To investigate the effect of transcription factor Slug silencing on the process of epithelial-mesenchymal transition (EMT) in prostate cancer cell line LNCaP after hypoxia and its related molecular mechanism. Methods: Slug silencing LNCaP cell line was established by shRNA transfection technique, and EMT was induced by hypoxia. Western blot was used to detect the expression of E-cadherin and N-cadherin in EMT. Transwell Invasion Assay to Evaluate Changes in Cell Invasion Ability. Results: Slug-shRNA stably transfected cell lines were successfully established. After hypoxia induction, the expression of E-cadherin in the control group was significantly decreased, while the expression of N-cadherin was significantly increased in the Slug-shRNA group Slug silencing inhibits hypoxia-induced increases in cell invasiveness. Conclusion: Slug gene silencing can inhibit EMT in hypoxia-induced prostate cancer cells. Slug is closely related to the metastasis of prostate cancer.