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目的和方法：研究重组人白细胞介素－ １３（ｒｈＩＬ－ １３） 对Ｄａｍｉ 细胞的作用，在血浆凝块培养基中分别加入ｒｈＩＬ－ １３ ５０ ｎｇ／ ｍＬ、１００ ｎｇ／ ｍＬ、１５０ ｎｇ／ ｍＬ，计数Ｄａｍｉ 细胞的集落数，在液体培养基中同样加入ｒｈＩＬ－ １３ ５０ ｎｇ／ ｍＬ、１００ｎｇ／ ｍＬ、１５０ ｎｇ／ｍ Ｌ，检测掺入到Ｄａｍｉ 细胞ＤＮＡ 中的５ － 溴－ ２ － 脱氧尿嘧啶（５ － ＢｒｄＵ） 。结果：血浆凝块培养基中加入ｒｈＩＬ－ １３ 能使Ｄａｍｉ 细胞扩增，５０ ｎｇ／ ｍＬ、１００ ｎｇ／ｍ Ｌ、１５０ｎｇ／ｍＬｒｈＩＬ－ １３ 实验组Ｄａｍｉ 细胞集落数分别是对照组的１ ．４２倍、１ ．８５ 倍、２ ．３１ 倍。５ － ＢｒｄＵ 掺入实验结果表明，ｒｈＩＬ－ １３ ５０ ｎｇ／ｍＬ、１００ ｎｇ／ ｍＬ、１５０ ｎｇ／ｍＬ 实验组的吸光度分别比对照组增加了４７ ．０６ ％ 、８８ ．２４ ％ 、１１４ ．７１ ％ 。结论：ｒｈＩＬ－ １３ 促进了Ｄａｍｉ 细胞的增殖。
Objective: To study the effect of recombinant human interleukin-13 (rhIL-13) on Dami cells. Add rhIL-13 50 ng / mL, 100 ng / mL, 150 ng / mL, The number of colonies of Dami cells was counted and rhIL-13 50 ng / mL, 100 ng / mL, and 150 ng / mL were also added to the liquid culture medium to detect 5-bromo-2-deoxyuracil (5 - BrdU). Results: The proliferation of Dami cells was induced by adding rhIL-13 to the plasma clotting medium. The numbers of Dami cells in the groups of 50 ng / mL, 100 ng / mL and 150 ng / mL rhIL-13 were respectively 1 in the control group. 42 times, 1. 85 times, 2. 31 times. The results of 5 - BrdU incorporation showed that the absorbance of rhIL-13 50 ng / mL, 100 ng / mL and 150 ng / mL experimental groups were increased by 47 compared with the control group respectively. 06%, 88. 24%, 114. 71%. Conclusion: rhIL-13 promotes the proliferation of Dami cells.