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目的检测1,25-二羟基维生素D_3[1,25(OH)_2D_3]对小鼠成骨细胞增殖及细胞周期的影响及其意义。方法取出生24 h内的小鼠30只,无菌条件下取出颅骨,应用酶消化法进行成骨细胞培养,在培养液中加入不同浓度的1,25(OH)_2D_3(10~(-8)、10~(-9)、10~(-11) mol/L),应用四唑蓝比色法(MTT)法检测其对成骨细胞增殖的影响,应用流式细胞仪检测其对成骨细胞细胞周期的影响。结果小鼠成骨细胞在1,25(OH)_2D_3真处理的24、48、72 h,10~(-8)、10~(-9) mol/L组与对照组吸光度(A)相比较,差异有统计学意义(P＜0．01),10~(-11) mol/L组与对照组A比较,差异无统计学意义(P＞0．05)；1,25(OH)_2D_3作用下小鼠成骨细胞S期(8．00±1．42)、G2-M期(7．70±0．67)的细胞减少,G1期(84．30±1．90)细胞增加。结论1,25(OH)_2D_3可以抑制体外培养的小鼠成骨细胞的增殖,并呈现一定的浓度依赖性。
Objective To investigate the effect of 1,25-dihydroxyvitamin D_3 [1,25 (OH) _2D_3] on the proliferation and cell cycle of mouse osteoblasts and its significance. Methods Thirty mice from 24 h after birth were removed and the skull was removed under sterile conditions. Osteoblasts were cultured by enzymatic digestion. Different concentrations of 1,25 (OH) _2D_3 (10 ~ (-8) ), 10 ~ (-9), 10 ~ (-11) mol / L). The effect of MTT on the proliferation of osteoblasts was detected by flow cytometry. Effect of osteocyte cell cycle. Results Compared with the control group, the osteoblasts in 24, 48, 72 h, 10 ~ (-8), 10 ~ (-9) mol / L treated with 1,25 (OH) (P <0.01). There was no significant difference between the 10 ~ (-11) mol / L group and the control group A (P> 0.05), but there was no significant difference between the 1,25 (OH) _2D_3 The number of cells in S phase (8.00 ± 1.42), G2-M phase (7.70 ± 0.67) and the G1 phase (84.30 ± 1.90) increased. Conclusion 1,25 (OH) _2D_3 can inhibit the proliferation of cultured mouse osteoblasts in a concentration-dependent manner.