应用电镜观察阴道毛滴虫(Trichomonas vaginalis)超微结构过去曾有报道,其中除透射电镜观察外,Warton(1979)、黄琼瑗等(1985)和杜之鸣等(1988)均作了扫描电镜的观察。我们最近也作了阴道毛滴虫的扫描电镜观察。材料和方法取滴虫性阴道炎患者的阴道分泌物,接种培养后,取培养液离心沉淀,清洗后经过戊二醛和锇酸双固定,梯度脱水,醋酸异戊酯置换,临界点干燥、喷金,用日立S—450扫描电镜观察。 The electron microscopic observation of Trichomonas vaginalis ultrastructure has been reported in the past, except for transmission electron microscopy, Warton (1979), Huang Qiongzuo et al (1985) and Du Zhiming et al (1988) were made by scanning electron microscopy Observed. We have also recently made a scanning electron microscopic observation of Trichomonas vaginalis. Materials and Methods Vaginal secretions from patients with trichomonas vaginitis were collected. After inoculation, the culture fluid was centrifuged, washed, and then fixed with glutaraldehyde and osmium tetroxide. Gradient dehydration, isoamyl acetate replacement, critical point drying, Spray gold, with Hitachi S-450 scanning electron microscopy.