论文部分内容阅读
目的从细胞水平上研究虾青素对丙烯酰胺所致海马神经细胞损伤恢复的影响。方法通过B27无血清培养法进行SD乳鼠的大脑海马神经细胞的分离、培养及其鉴定;MTT法检测细胞存活率,比色法测定细胞内抗氧化酶SOD、CAT、GSH-Px的活性,线粒体的MDA及NO的含量。结果虾青素能提高丙烯酰胺损伤过的海马神经细胞的存活率,提高了丙烯酰胺损伤后细胞SOD、CAT活性、GSH-Px的活力,并降低了细胞线粒体中的MDA和NO的含量。结论虾青素对丙烯酰胺造成的高海马神经细胞损伤有促进恢复的作用。
Objective To study the effect of astaxanthin on the recovery of the damage of hippocampal neurons induced by acrylamide on the cellular level. Methods The B27 serum-free culture method was used to isolate, culture and identify the hippocampal neurons of SD neonatal rats. The cell viability was measured by MTT assay. The activities of intracellular antioxidant enzymes SOD, CAT and GSH-Px were determined by colorimetric assay. Mitochondrial MDA and NO content. Results Astaxanthin could increase the survival rate of hippocampal neurons damaged by acrylamide and increase the activity of SOD and CAT, the activity of GSH-Px and the content of MDA and NO in mitochondria after acrylamide injury. Conclusion Astaxanthin can promote the recovery of hippocampal nerve cell injury caused by acrylamide.