论文部分内容阅读
为明确中国河南省小麦纹枯病菌对苯醚甲环唑及戊唑醇的敏感性,采用菌丝生长速率法测定了2种药剂对采自河南省15个地市分离的95株病菌的毒力。结果显示:供试菌株对苯醚甲环唑和戊唑醇的EC_(50)值分别在0.014~1.609和0.002~0.064μg/m L之间。敏感性频率分布图显示:在小麦纹枯病菌群体中已出现对苯醚甲环唑敏感性下降的亚群体,而对戊唑醇则不明显;可将其中呈连续单峰频次分布的大多数菌株群体的平均EC_(50)值(0.165±0.101)和(0.019±0.008)μg/m L分别作为小麦纹枯病菌对苯醚甲环唑和戊唑醇的相对敏感基线。苯醚甲环唑的平均EC_(50)值是戊唑醇的26倍,表明该病原菌对戊唑醇更为敏感。不同地区病原菌群体间对苯醚甲环唑和戊唑醇的敏感性均存在显著性差异。小麦纹枯病菌对2种杀菌剂的敏感性之间存在着微弱的正相关性。本研究结果可为河南省小麦纹枯病防控杀菌剂的合理使用提供理论依据。
In order to make clear the sensitivity of difenoconazole to difenoconazole and tebuconazole in Henan Province in China, the mycelium growth rate method was used to determine the toxicity of 95% of the two bacteria isolated from 15 municipalities in Henan Province Toxicity. The results showed that the EC 50 values of difenoconazole and tebuconazole were 0.014-1.609 and 0.002-0.064 μg / m L, respectively. The frequency distribution of sensitivity showed that there was a subpopulation of wheat diflubenzuron susceptible to difenoconazole but not to tebuconazole; The average EC 50 values of most strains (0.165 ± 0.101) and (0.019 ± 0.008) μg / m L were the sensitive baseline for difenoconazole for difenoconazole and tebuconazole, respectively. Difenoconazole had an average EC 50 value 26 times that of tebuconazole, indicating that the pathogen is more sensitive to tebuconazole. The sensitivity of difenoconazole and tebuconazole between different populations of pathogenic bacteria were significantly different. There is a weak positive correlation between the susceptibility of R. solani to the two fungicides. The results of this study may provide a theoretical basis for the rational use of fungicides for the control of wheat sheath blight in Henan Province.