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目的 探讨细胞核显微注射导入外源基因的方法 ,观察 HBV- S基因导入人肝癌细胞株 SMMC- 772 1后的表达及其稳定性 .方法 将含有 HBV- S片段的质粒 p CR3.1- S线性化 ,通过显微注射仪直接注入培养的 SMMC- 772 1细胞核内 ,G418筛选后 ,经EIA和免疫荧光法分别检测细胞培养上清
Objective To investigate the method of microinjection of exogenous gene into nucleus and observe the expression and stability of HBV-S gene in human hepatocellular carcinoma cell line SMMC-772 1.Methods The plasmid pCR3.1-S containing HBV-S fragment Linearized and directly injected into the nucleus of SMMC-772 1 cells by microinjection. After G418 selection, the cell culture supernatants were detected by EIA and immunofluorescence