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目的:观察micro RNA-134(miR-134)在缺氧预处理新生大鼠缺氧缺血后不同时期脑组织中的表达差异及其表达的特点及意义。方法:采用7日龄SD大鼠制备新生大鼠缺血缺氧性脑损伤动物模型。完全随机分为单纯缺血缺氧组、假手术对照组、缺氧预处理组(各18只)。3组又各分为处理后0h、1d、7d组(n=6)。每组6只用于荧光实时定量聚合酶链反应(qRT-PCR)观察miR-134转录量的差异。结果:miR-134在各组脑组织转录的表达量,单纯缺血缺氧组0 h(5.061±0.761)、1d(4.120±0.685)、7d(2.873±0.397);缺氧预处理组0 h(3.341±0.575)、1 d(2.769±0.351)、7d(1.658±0.290);假手术组0 h(6.617±1.988)、1d(5,798±1.116)、7d(5.984±1.879)。miR-134在单纯缺血缺氧组及缺氧预处理组脑组织的表达显著比假手术组减少(P<0.01),在同一时间段上,缺氧预处理组比单纯缺血缺氧组miR-134的表达明显减少(P<0.01)。同样在缺血缺氧后,无论是单纯缺血缺氧组还是缺氧处理组,miR-134的表达随着0h、1、7d的推移表达渐减少(P<0.05)。结论:miR-134的表达在调控神经系统发育中可能起着重要作用,缺氧预处理对新生大鼠缺血缺氧性脑损伤存在保护作用。
Objective: To observe the difference and expression of micro RNA-134 (miR-134) in different stages of hypoxic-ischemic preconditioning hypoxic-ischemic brain damage. Methods: 7-day-old SD rats were used to prepare neonatal rat models of hypoxic-ischemic brain damage. Completely randomized into simple hypoxic-ischemic group, sham-operated control group, hypoxic preconditioning group (18 each). Three groups were divided into treatment 0h, 1d, 7d group (n = 6). Six mice in each group were used for real time quantitative PCR (qRT-PCR) to observe the difference of miR-134 transcription. Results: The expression of miR-134 in the brain tissue of each group was significantly higher than those in the hypoxia preconditioning group at 0 h (5.061 ± 0.761), 1d (4.120 ± 0.685), 7d (2.873 ± 0.397) (3.341 ± 0.575), 1 d (2.769 ± 0.351) and 7d (1.658 ± 0.290) respectively. The sham-operated group had no significant difference between the sham-operation group and the sham-operated group for 0 h (6.617 ± 1.988), 1d (5,798 ± 1.116) and 7d (5.984 ± 1.879). The expression of miR-134 in the brain tissue of hypoxia-deficient group and hypoxic preconditioning group was significantly lower than that of the sham operation group (P <0.01). Compared with the hypoxia-ischemia group The expression of miR-134 was significantly decreased (P <0.01). Similarly, after ischemia and hypoxia, the expression of miR-134 decreased gradually (P <0.05) at 0h, 1d and 7d, both in hypoxia-ischemia and hypoxia groups. CONCLUSION: The expression of miR-134 may play an important role in the regulation of nervous system development. Hypoxic preconditioning may protect hypoxic-ischemic brain damage in neonatal rats.