论文部分内容阅读
目的识别和确定与广西巴马地区长寿人群相关的基因。方法采用长寿对照方法,分组:129名年龄(94～113)岁的长寿者为长寿组,长寿者家人年龄不大于 59岁的 179人为年龄对照组,当地非长寿自然人群153人作为自然人群对照组,进行ApoE基因分型,分型方法采用PCR-RFLP,并经测序确定。分别比较长寿组与各组人群的基因型、基因频率的差别、并进行显著性检验。各个对照组的ApoE基因频率均符合Hardg—Weinberg平衡。结果①长寿组与其亲属年龄对照的ApoE基因型和基因频率相比之间均无显著性差别,表明两者属同一人群,且年龄变化与基因频率无关。②长寿组与当地自然人群对照组相比较有显著性阳性关联(P=0.0001,P=0.04)的为ε2/ε2、ε3/ε3基因型。其中ε3/ε3纯合子是最主要的长寿关联基因。
Objective To identify and identify the genes related to longevity in Guangxi Bama region. Methods: The longevity control group was divided into two groups: 129 longevity aged 94--113 years old were longevity group, 179 elderly people whose longevity was no older than 59 years old were age control group, and 153 non-longevity natural people were taken as control group Group, ApoE genotyping, typing method using PCR-RFLP, and determined by sequencing. The differences of genotypes and gene frequencies between the longevity group and the groups were compared and the significance test was carried out. The ApoE gene frequencies of all the control groups were in line with the Hardg-Weinberg equilibrium. Results ① There was no significant difference in age-related ApoE genotype and gene frequency between the longevity group and its relatives, indicating that the two groups were in the same population with no relationship between age and gene frequency. ② There was a significant positive correlation (P = 0.0001, P = 0.04) between the longevity group and the local control group for the ε2 / ε2 and ε3 / ε3 genotypes. Which ε3 / ε3 homozygotes is the most important longevity associated genes.