应用自制的磺胺二甲嘧啶(SM_2)-蛋白质偶联物,经免疫得高质量兔抗SM_2抗血清。建立了测定SM_2的酶联免疫吸附分析(ELISA)竞争法,重现性好,特异性强,测定线性范围0.05～5.0μg·L~(-1),最低检出浓度为0.025μg·L~(-1)。全脂牛奶与脱脂牛奶的加标实验回收率分别为82.1％～128.8％和90.6％～124.0％。测定了270个牛奶试样,未发现大于欧盟允许的最高浓度25μg·L~(-1)的阳性试样。 Self-made sulfadimidine (SM_2) -protein conjugates were used to produce high quality rabbit anti-SM 2 antiserum. A competitive ELISA method was established for the determination of SM_2. The reproducibility was good and the specificity was good. The linear range was 0.05-5.0 μg · L -1, and the lowest detection limit was 0.025 μg · L ~ (-1). The spiked recoveries of whole milk and skim milk were 82.1% ~ 128.8% and 90.6% ~ 124.0%, respectively. A total of 270 milk samples were determined and no positive samples greater than 25 μg · L -1 maximum allowable by the EU were found.