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采用Ｃｕ２＋在体外氧化极低密度脂蛋白（ＶＬＤＬ），用甲基莲心碱（Ｎｅｆ）作抗氧化剂。将脂蛋白分为正常、氧化和Ｎｅｆ抗氧化三组，测定脂蛋白的硫代巴比妥酸反应物质（ＴＢＡＲＳ）。结果分别为（０．６１±０．０３）、（６．０８±０．２５）和（２．３３±０．９１）μｍｏｌ／ｇ蛋白，每二组间的差异均有显著性（Ｐ＜０．０１）。氧化和Ｎｅｆ抗氧化的ＶＬＤＬ与巨噬细胞（ＭΦ）在３７℃温育４８ｈ后，ＭΦ内甘油三酯（ＴＧ）和总胆固醇（ＴＣ）含量显示前者比后者有更强的促进ＭΦ内脂质堆积的作用（Ｐ＜０．０５）。研究结果表明，Ｎｅｆ能够有效抑制Ｃｕ２＋介导的ＶＬＤＬ的氧化修饰及ＭΦ源性泡沫细胞的形成
Cu2+ was used to oxidize Very Low Density Lipoprotein (VLDL) in vitro and nefolid (Nef) was used as an antioxidant. The lipoproteins were divided into three groups: normal, oxidized, and Nef antioxidative, and thiobarbituric acid-reactive substances (TBARS) of lipoprotein were measured. The results were (0.61±0.03), (6.08±0.25), and (2.33±0.91) μmol/g protein, respectively, and the difference between the two groups was significant (P< 0.01). After incubation of oxidized and Nef anti-oxidized VLDL with macrophages (MΦ) for 48 h at 37°C, the contents of triglyceride (TG) and total cholesterol (TC) in MΦ showed that the former had stronger promotion of MΦ lactone than the latter. The role of mass accumulation (P<0.05). The results show that Nef can effectively inhibit Cu2+-mediated oxidative modification of VLDL and the formation of MΦ-derived foam cells.