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目的 :探讨肿瘤坏死因子α (tumornecrosisfactorα ,TNF α)对晶状体上皮细胞在细胞外基质上的粘附移行作用 ,以及在此过程中整合素分子的表达情况。方法 :借助细胞粘附实验和移行实验研究TNF α对体外培养的牛晶状体上皮细胞在细胞外基质上的粘附和移行 ,以及整合素抗体封闭后对细胞的影响。TNF α对细胞表面整合素的表达调节经免疫荧光染色后由流式细胞仪检测。结果 :TNF α可以明显促进晶状体上皮细胞在Ⅳ型胶原、层粘蛋白、纤维连接蛋白上的粘附移行。抗体封闭实验表明整合素 β1、α2、α3、α5亚基参与细胞的粘附 ,在细胞移行中发挥主要作用的是α亚基而非 β亚基。流式细胞仪检测表明TNF α可上调晶状体上皮细胞中整合素的表达。结论 :胶原、层粘蛋白、纤维连接蛋白是晶状体囊的主要成分 ,肿瘤坏死因子α促进晶状体上皮细胞与晶状体囊膜成分的粘附移行 ,该作用的发挥至少部分通过TNF α上调整合素分子在细胞上的表达而实现
Objective: To investigate the adhesion and migration of tumor necrosis factor α (TNF α) to extracellular matrix of lens epithelial cells and the expression of integrin molecules in this process. Methods: Cell adhesion and migration experiments were performed to study the effect of TNFα on the adhesion and migration of cultured bovine lens epithelial cells in extracellular matrix. The regulation of cell surface integrin expression by TNFα was detected by flow cytometry after immunofluorescence staining. Results: TNFα significantly promoted the adhesion and migration of lens epithelial cells on type Ⅳ collagen, laminin and fibronectin. Antibody blocking experiments show that integrin β1, α2, α3, α5 subunits involved in cell adhesion, play a major role in cell migration α subunit rather than β subunit. Flow cytometry showed that TNFα upregulated integrin expression in lens epithelial cells. CONCLUSIONS: Collagen, laminin and fibronectin are the main components of the lens capsule. Tumor necrosis factor-α promotes the adhesion and migration of lens epithelial cells and lens capsule components. The effect of TNFα at least partly up-regulates integrin molecules in Cells on the expression and achieve