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目的通过对结肠癌及正常结肠黏膜组织标本中maspin和uPA基因的表达进行检测,探讨二者与结肠癌病理学特征之间的关系,以及在组织中表达的相关性,为临床寻找结肠癌早期诊断指标提供理论依据。方法采用免疫组织化学SP法测定62例结肠癌及30例正常结肠组织中maspin以及uPA基因的表达,对结果进行分析。结果 maspin蛋白的阳性表达率在结肠癌未侵及浆膜、淋巴结无转移及TNM分期0～Ⅰ期者中明显高于侵及浆膜、淋巴结有转移及TNM分期Ⅱ～Ⅳ期者(P均<0.05),在结肠癌不同性别患者中无差异(P>0.05)。uPA蛋白的阳性表达率在结肠癌未侵及浆膜、淋巴结无转移及TNM分期0～Ⅰ期者中明显低于侵及浆膜、淋巴结有转移及TNM分期Ⅲ～Ⅳ期者(P均<0.05),在结肠癌不同性别患者中亦无差异(P>0.05)。maspin与uPA基因在结肠癌的表达中呈负相关(rs=-0.416,P<0.05)。结论 maspin和uPA基因在结肠癌和正常结肠黏膜组织中的表达有明显差异,且与结肠癌的病理学特征相关,二者在结肠癌组织样本中的表达也存在相关性,可作为反映结肠癌病理生物学行为的指标。
OBJECTIVE: To detect the expression of maspin and uPA in colon and normal colonic mucosa tissues and to explore the relationship between them and the pathological features of colon cancer and the correlation between them in tissues. Diagnostic indicators provide a theoretical basis. Methods Immunohistochemical SP method was used to detect the expression of maspin and uPA in 62 cases of colon cancer and 30 cases of normal colon tissues. The results were analyzed. Results The positive expression rate of maspin protein in colon cancer without invasion of serosa, lymph node metastasis and TNM staging stage 0 ~ Ⅰ was significantly higher than that in serosal invasion, lymph node metastasis and stage Ⅱ ~ Ⅳ TNM stage (P <0.05), there was no difference in patients with different sexes of colon cancer (P> 0.05). The positive expression rate of uPA protein in colon cancer without invasion of serosa, lymph node metastasis and TNM staging stage 0 ~ Ⅰ was significantly lower than that of serosal invasion, lymph node metastasis and TNM staging Ⅲ ~ Ⅳ (P < 0.05). There was no difference in patients with different sexes of colon cancer (P> 0.05). There was a negative correlation between maspin and uPA expression in colon cancer (rs = -0.416, P <0.05). Conclusion The expressions of maspin and uPA in colorectal cancer and normal colorectal mucosa are significantly different, and are correlated with the pathological features of colon cancer. The expression of both maspin and uPA in colon cancer tissues is also correlated with the expression of colon cancer Pathobiological behavior indicators.