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以多子芋品种‘香堂芋’幼嫩叶片为试材,利用RACE结合RT-PCR技术克隆得到全长为3 051 bp的淀粉分支酶SBE基因c DNA序列(登录号:KX013544),其中开放阅读框长2 538 bp,编码845个氨基酸;该序列与玉米SBEII、马铃薯SBEIIa同源性均达85%以上;芋SBE编码蛋白具有N–末端、C–末端和α–淀粉酶催化域。利用RT-PCR技术克隆得到芋SBE的基因组DNA序列,全长为12362 bp,包括20个外显子和19个内含子。芋球茎形成过程中,子芋植株叶片中SBE表达量显著高于母芋植株叶片、叶柄和根系及母芋等组织,子芋膨大过程中SBE表达量逐渐升高,而孙芋膨大过程中SBE表达量逐渐下降。子芋、孙芋SBE表达对支链淀粉合成积累可能起重要作用。
Using the young leaves of more than sub-taro variety Xiangxiangluo as test material, the cDNA sequence of SBE gene of starch branching enzyme with a total length of 3 051 bp (accession number: KX013544) was cloned by RACE and RT-PCR technology. Among them, The reading frame is 2 538 bp in length and encodes 845 amino acids. The homology of this sequence with that of both maize SBEII and potato SBEIIa is above 85%. The SBE encoded protein has N-terminal, C-terminal and α-amylase catalytic domains. The genomic DNA sequence of SBE was cloned by RT-PCR. The full-length cDNA was 12362 bp, including 20 exons and 19 introns. During the process of taro corm formation, the expression level of SBE in the leaves of sub-taro plants was significantly higher than that in the leaves, petioles and roots of the parent taro plants and the parental roots. The expression of SBE increased gradually during the expansion of the taro roots. However, The amount of expression gradually decreased. Sub-taro, taro SBE expression accumulation of amylopectin may play an important role.