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以三倍体毛白杨叶片为外植体,筛选适宜遗传转化叶片的诱导分化培养基和生根培养基,确定转化体最佳选择压浓度和抗生素浓度。结果表明:叶片诱导分化培养基以MS+6-BA 0.6 mg/L+NAA 0.1 mg/L附加1.5%蔗糖、0.6%琼脂效果最好,最佳生根培养基为1/2MS基本培养基附加IBA 0.4 mg/L、1.5%蔗糖、0.6%琼脂。选择压浓度以50 mg/L卡那霉素对叶片分化和嫩茎生根效果显著。培养基中附加300 mg/L的羧卞青霉素不仅有效的抑制农杆菌的生长,而且还对分化起促进作用。
Triploid populus tomentosa leaves were used as explants to screen the appropriate differentiation medium and rooting medium for genetic transformation leaves to determine the optimum selection pressure of transformants and antibiotic concentration. The results showed that 1.5% sucrose was supplemented with 0.6 mg / L MS + 6-BA 0.6 mg / L and NAA 0.1 mg / L, and 0.6% agar was the best medium for inducing differentiation in leaves. IBA 0.4 mg / L, 1.5% sucrose, 0.6% agar. Select pressure concentration of 50 mg / L kanamycin on leaf differentiation and tender stem rooting effect is significant. The addition of 300 mg / L carbenicillin to the medium not only effectively inhibited the growth of Agrobacterium, but also promoted the differentiation.