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目的用改进的Loewe等效线模型法计算盐霉素和索拉非尼合用抑制人肺腺癌细胞A549生长的协同、相加和拮抗浓度范围。方法 MTT法检测药物对肿瘤细胞生长的抑制率,用Excel和ORIGIN 8.0进行数据处理,拟合得出单用药组和合用药组量效曲线及函数方程,计算在ED_(30)、ED_(50)、ED_(70)、ED_(90)时的合用指数。用合用组的量效曲线方程计算不同药物浓度水平合用时对应的抑制率,并分别代入各单药组量效曲线方程式,计算出相应的单用药物浓度值以及合用组各浓度水平的合用指数值,重构合用组药物浓度与合用指数值关系曲线图,并计算确定两药合用时协同、相加和拮抗效应的浓度范围。结果及结论在盐霉素+索拉非尼3种浓度比例合用的3种方式下,在ED_(30)、ED_(50)、ED_(70)、ED_(90)时,3种合用方式均体现为相加效应特征。按照本方法可以计算得出不同合用方式的相加、拮抗和协同的药物浓度范围,有利于帮助选择适当的合用比例。
OBJECTIVE: To evaluate the synergistic, additive and antagonistic concentrations of salinomycin and sorafenib in inhibiting the growth of human lung adenocarcinoma A549 cells by a modified Loewe’s equivalent line model. Methods MTT method was used to detect the inhibitory rate of tumor growth. The data were processed with Excel and ORIGIN 8.0. The dose-response curves and function equations of single drug group and combined drug group were obtained. The data of ED_ (30), ED_ (50) , ED_ (70), ED_ (90) combined index. The dose-effect curve equation of the combined group was used to calculate the corresponding inhibition rate when the different drug concentration levels were combined, and the dose-effect curve equations of each single drug group were respectively substituted to calculate the corresponding single drug concentration value and the combination index Values, reconstructed the combination group drug concentration and co-index value curve, and calculate the concentration of synergy, additive and antagonistic effects of the two drugs to determine the concentration range. RESULTS AND CONCLUSION Three combinations of salinomycin and sorafenib at the three concentrations of ED30, ED50, ED70 and ED90, Reflected as the additive effect characteristics. According to the method, the additive, antagonistic and synergistic drug concentration ranges of different combination modes can be calculated, which helps to select the appropriate combination proportion.