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目的 观察同型半胱氨酸是否改变体外健康人血单核细胞、中性粒细胞及淋巴细胞表面粘附分子CD11b ,CD18,CD14和L 选择素 (L selectin)的表达。方法 细胞表面粘附分子CD11b ,CD18,CD14和L selectin的测定采用直接免疫荧光染色 ,2 4小时内用流式细胞仪测定。结果 同型半胱氨酸在低浓度 (2 0 μmol/L)时明显增加CD11b和CD18在各种白细胞表面的表达 ,同时也增加了CD14在单核及中性粒细胞的表达。这种作用随同型半胱氨酸浓度升高而增强 ,但当浓度升至≥ 1mmol/L时 ,各种粘附分子的表达反而降低。同型半胱氨酸使各种细胞类型表面L selectin降低。结论 同型半胱氨酸可改变白细胞表面粘附分子的表达 ,可能在体内通过此途经导致白细胞粘附并游出于血管内皮。
Objective To investigate whether homocysteine can change the expression of CD11b, CD18, CD14 and L selectin on human monocytes, neutrophils and lymphocytes in vitro. Methods The cell adhesion molecules CD11b, CD18, CD14 and L selectin were determined by direct immunofluorescence staining and determined by flow cytometry in 24 hours. Results Homocysteine significantly increased the expression of CD11b and CD18 on various leukocytes at low concentration (20 μmol / L), and also increased the expression of CD14 on monocytes and neutrophils. This effect increased with increasing homocysteine concentration, but when the concentration rose to ≥ 1 mmol / L, the expression of various adhesion molecules decreased. Homocysteine reduces L selectin on the surface of various cell types. Conclusion Homocysteine can change the expression of adhesion molecules on the surface of leukocytes and may lead to leukocyte adhesion and migration to the vascular endothelium through the process.