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探讨中药热毒清抗人巨细胞病毒（ＨＣＭＶ）的临床疗效及体外抗病毒作用。方法：临床检测ＨＣＭＶ抗体，对其中诊断为ＨＣＭＶ活动性感染者１４例采用热毒清口服治疗，疗程１８～３０天；同时应用ＥＬＩＳＡ、ＰＣＲ和贝利婴幼儿发育量表等方法评价其临床疗效。体外实验研究采用病变抑制法在人胚肺（ＨＥＬ）细胞培养上测定热毒清对ＨＣＭＶ ＡＤ１６９毒株的抑制作用。结果：１４例孕前或孕期活动性感染者，服药１８～３０天后，ＨＣＭＶ－ＩｇＭ全部转阴（１４／１４），血ＨＣＭＶ ＤＮＡ大部分转阴（７／１０），尿和宫颈分泌物排毒被抑制（４／４，１／１）。其中５例孕前或孕期治疗后分娩足月正常新生儿，２例为无症状排毒新生儿，３例未感染。体外实验研究结果：热毒清的最大无毒浓度（ＴＤ０）为２０μｇ／Ｌ，最小有效浓度（ＭＴＣ）为 ５μｇ／Ｌ，治疗指数（ＴＩ）为４。表明热毒清具有抗ＨＣＭＶ作用，随药物浓度增高作用增强。结论：中药热毒清是抗ＨＣＭＶ安全、有效的药物，为临床治疗ＨＣＭＶ活动性感染尤其是孕期感染提供了有效的参考。
To investigate the clinical efficacy of anti-human cytomegalovirus (HCMV) and its antiviral effect in vitro. METHODS: Clinical detection of HCMV antibody was performed in 14 cases with active HCMV infection. The treatment was performed with fluoxetine orally for 18 to 30 days. Clinical efficacy was evaluated by ELISA, PCR, and Bely infant developmental scales. . In vitro studies were performed to determine the inhibitory effect of heat toxin on HCMV AD169 strains in human embryo lung (HEL) cell cultures using lesion inhibition assays. Results: Fourteen patients with active infection before pregnancy or during pregnancy, 18 to 30 days after taking the drug, all HCMV-IgM negative (14/14), most of the blood HCMV DNA negative (7/10), urine and cervical secretions were detoxified Suppression (4/4, 1/1). Five of them were normal neonates who delivered their babies before pregnancy or during pregnancy, two were asymptomatic detoxification neonates, and three were uninfected. In vitro studies showed that the maximum non-toxic concentration (TD0) of DHQ was 20 μg/L, the minimum effective concentration (MTC) was 5 μg/L, and the therapeutic index (TI) was 4. It was shown that DHHP has the effect of anti-HCMV, and it increases with the increase of drug concentration. Conclusion: The Chinese herbal medicine Duduqing is a safe and effective anti-HCMV drug, which provides an effective reference for clinical treatment of HCMV active infections, especially during pregnancy.