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目的:探讨异体同型输血的科学检测方法。方法:将若干份单一血液按比例实施体外两两混合,分别采用流式细胞的免疫血液学检测法及PCR-STR分析技术检测样本是否为混合血。结果:流式细胞仪检测法检测205份有效混合血样,检测出为混合血样的198份,其中2例为真正的输血病人,疑似混合血样品7份,无假阴性报告;相对于洛桑的8种目标检测抗原,采用21种目标检测抗原提高了确诊率。对291份有效混合血样采用PCR-STR分析技术进行检测,结果确定为混合血样的240份,其中1份为真正的输血病人,疑似混合血样39份,其中1份为输血病人,未检测出混合血迹象的12份;当血样混合比例高于1:10时,确诊率才明显下降。结论:在现阶段,流式细胞仪检测法和PCR-STR分析法是可行的异体同型输血检测方法。
Objective: To explore the method of scientific detection of allogeneic blood transfusion. Methods: A number of single blood in proportion to the implementation of two or two in vitro mixed, respectively, using flow cytometry and PCR-STR analysis of the immune test samples were mixed blood. RESULTS: A total of 198 validated mixed blood samples were detected by flow cytometry and 198 were detected as mixed blood samples, of which 2 were true transfusion patients and 7 suspected mixed blood samples without false negative reports. The target detection antigen, using 21 kinds of target detection antigen to improve the diagnosis rate. Of 291 validated mixed blood samples, PCR-STR analysis was used to determine 240 mixed blood samples, of which 1 was true blood transfusion and 39 suspected mixed blood samples, 1 of which was a transfusion patient and no detectable mixed 12 cases of blood signs; when the blood sample mixture ratio higher than 1:10, the diagnosis rate was significantly decreased. CONCLUSION: At this stage, flow cytometry and PCR-STR analysis are feasible methods of allogeneic blood transfusion testing.