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为研究不同类型胡麻种质萌发期抗旱性表现,以筛选抗旱品种,为抗旱育种提供科技支撑。本研究采用不同浓度的PEG-6000高渗溶液模拟干旱胁迫的方法,对定亚17、陇亚8号和2011621080进行渗透胁迫试验,筛选适宜浓度的PEG-6000溶液。对161份不同类型的胡麻种质在萌发期进行渗透胁迫试验,考查与抗旱性相关的5个性状,并运用单项抗旱系数、综合抗旱系数、隶属函数和灰色关联度相结合的方法对种质资源的抗旱性进行综合评价。结果表明,不同浓度PEG-6000胁迫对胡麻种质的萌发均有影响,当PEG-6000的浓度在18%时,各种指标的抑制作用均显著加强,因此本研究选用18%的PEG-6000浓度模拟干旱胁迫试验;PEG-6000胁迫降低了161份胡麻种质资源的相对发芽率,抑制了幼芽的生长,种质间表现出较大差异。根据抗旱性量度值将供试种质划为5级,其中1级抗旱型2份、2级15份、3级28份、4级82份、5级34份;供试种质的抗旱性强弱与地理来源息息相关。试验结果说明选择多个性状,综合评价胡麻萌发期抗旱性是可行且准确的。
In order to study the drought resistance of different types of flax germplasm germination, screening drought resistant varieties and providing scientific and technological support for drought resistance breeding. In this study, different concentrations of PEG-6000 hypertonic solution to simulate the drought stress method, Dingya 17, Longya 8 and 2011621080 osmotic stress test, screening the appropriate concentration of PEG-6000 solution. Sixty-one varieties of flax germplasm were subjected to osmotic stress during the germination period. Five traits related to drought resistance were investigated. The germplasm of germplasm was screened by combining single drought resistance coefficient, comprehensive drought resistance coefficient, membership function and gray correlation degree Resource drought resistance comprehensive evaluation. The results showed that different concentrations of PEG-6000 had effects on the germination of flax germplasm. When the concentration of PEG-6000 was 18%, the inhibitory effects of various indexes were significantly enhanced. Therefore, 18% PEG-6000 Concentration simulated drought stress; PEG-6000 stress reduced the relative germination rate of 161 flax germplasm resources, inhibited the growth of young shoots, and showed significant differences among germplasms. According to the measurement of drought resistance, the quality of the tested cultivars was classified into 5 grades, of which 1 was drought-tolerant in 2, 15 in 2, 28 in 3, 82 in 4 and 34 in 5; Strength and geographical origin are closely related. The test results show that it is feasible and accurate to select multiple traits and comprehensively evaluate the drought resistance of flax during germination.