【摘 要】
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Autophagy has been shown to play an important role in Parkinson\'s disease.We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson\'s disease through affecting autophagy.In this study,6-hydroxydopamine-damaged SH
【机 构】
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Department of Neurology,Affiliated Hospital of Nantong University,Nantong,Jiangsu Province,China;Res
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Autophagy has been shown to play an important role in Parkinson\'s disease.We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson\'s disease through affecting autophagy.In this study,6-hydroxydopamine-damaged SH-SY5Y cells were pretreated with a culture medium containing skin-derived precursors differentiated into Schwann cells(SKP-SCs).The results showed that the SKP-SC culture medium remarkably enhanced the activity of SH-SY5Y cells damaged by 6-hydroxydopamine,reduced excessive autophagy,increased tyrosine hydroxylase expression,reduced α-synuclein expression,reduced the autophagosome number,and activated the PI3K/AKT/mTOR pathway.Autophagy activator rapamycin inhibited the effects of SKP-SCs,and autophagy inhibitor 3-methyladenine had the opposite effect.These findings confirm that SKP-SCs modulate the PI3K/AKT/mTOR pathway to inhibit autophagy,thereby exhibiting a neuroprotective effect in a cellular model of Parkinson\'s disease.This study was approved by the Animal Ethics Committee of Laboratory Animal Center of Nantong University(approval No.S20181009-205)on October 9,2018.
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