作者在丹麦NOVO公司制备了5种抗牛胰岛素McAb,并对其特点及实际应用进行了初步研究。结果表明,腹水液抗体滴度为2×10~3～2×10~5,免疫球蛋白含量高达10～30mg/ml,除1种为IgG_(2α)外,其余均属IgG1。不同的McAb,其半最大结合~(125)碘-牛胰岛素时间及半离解时间常数相差悬殊,因而与牛胰岛素结合的亲和力也很不一样(Ka为1.8×10~8～3.1×10~9 L/M)。不同种属的胰岛素原、胰岛素及其碎片的置换反应结果表明,5种 The authors prepared five anti-bovine insulin McAbs at NOVO in Denmark and conducted a preliminary study on their characteristics and practical application. The results showed that ascites fluid antibody titer of 2 × 10 ~ 3 ~ 2 × 10 ~ 5, immunoglobulin content as high as 10 ~ 30mg / ml, except for one species of IgG_ (2α), the rest are IgG1. Different McAb, the half-maximum combined ~ (125) iodine - bovine insulin time and semi-dissociation time constant difference between the very poor, and thus the affinity of bovine insulin is also very different (Ka 1.8 × 10 ~ 8 ~ 3.1 × 10 ~ 9 L / M). Different species of proinsulin, insulin and fragment replacement reaction results show that 5 species