论文部分内容阅读
目的 :探讨血管紧张素转换酶 (angiotensinconvertingenzyme ,ACE)基因多态性与 2型糖尿病合并颈动脉硬化性病变之间的关系。方法 :应用聚合酶链反应技术检测 176名 2型糖尿病患者ACE基因 16内含子插入 /缺失(insertion/deletion ,I/D)型多态性 ,利用B型超声检测糖尿病患者颈动脉硬化性病变的情况 ,通过Logistic多元回归分析 ,筛选 2型糖尿病患者颈动脉硬化性病变的危险因素。结果 :(1)糖尿病颈动脉硬化性病变组ACE基因DD型和D等位基因频率显著高于无颈动脉硬化性病变组 (DD基因型频率 :0 .5 0vs 0 .18;D等位基因频率 :0 .71vs0 .38;P <0 .0 1)。 (2 )Logistic回归分析显示 ,ACE基因DD型、年龄、合并高血压及男性是糖尿病颈动脉硬化性病变的危险因素 (OR分别为 3 .86 9,1.0 81,2 .44 7,2 .173,P <0 .0 5 )。 (3)在控制了年龄、性别、高血压后的危险性分层分析显示 ,ACE基因DD型是颈动脉硬化性病变的独立危险因素。结论 :ACE基因I/D多态性与 2型糖尿病颈动脉硬化性病变的发病相关 ,且其作用独立于年龄、性别及高血压病史
Objective: To investigate the relationship between angiotensin-converting enzyme (ACE) gene polymorphism and type 2 diabetes with carotid atherosclerosis. Methods: ACE gene 16 intron insertion / deletion polymorphism (I / D) polymorphism was detected in 176 patients with type 2 diabetes by polymerase chain reaction (PCR), and carotid atherosclerotic lesions The risk factors of carotid arteriosclerosis in type 2 diabetic patients were screened by Logistic multiple regression analysis. Results: (1) The frequencies of DD and D alleles of ACE gene in diabetic patients with carotid atherosclerosis were significantly higher than those without carotid arteriosclerosis (DD genotype frequency: 0.5 0 vs 0.18; D allele Frequency: 0 .71 vs 0 .38; P <0 .0 1). (2) Logistic regression analysis showed that ACE gene type DD, age, hypertension and males were the risk factors of carotid atherosclerosis in diabetic patients (OR = 3.86 9,1.081,2 .44 7,2,173 , P <0. 05). (3) Stratified analysis of risk stratified by age, gender and hypertension showed that ACE gene DD was an independent risk factor for carotid artery atherosclerosis. Conclusion: The ACE gene I / D polymorphism is associated with the pathogenesis of carotid atherosclerotic lesions in type 2 diabetes mellitus, and its effect is independent of the age, sex and history of hypertension