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体外试验表明青霉素和万古霉素对中度耐β—内酰胺和高度耐糖肽肠球菌有抗菌协同作用,作者选用屎肠球菌D400耐药菌株通过体外及体内试验评价该协同作用在动物体内是否也存在,该作用是否又可被庆大霉素加强。方法1.杀菌曲线:接种对数生长期细菌5×10~7cfu/ml至BHI肉汤中测试体外杀菌率。选择与各种给药方案兔血清中药物浓度相当的抗生素浓度,青霉素为2和40μg/ml,万古霉素为10μg/ml,庆大霉素为4μg/ml。于0、3、6、24h分别取样经系列稀释及
In vitro tests showed that penicillin and vancomycin have an antibacterial synergistic effect on moderately resistant β-lactams and highly resistant to glycopeptide enterococci. The authors selected Enterococcus faecium D400-resistant strains to evaluate whether this synergistic effect in animals is also demonstrated by in vitro and in vivo tests Existence, whether this effect can be strengthened by gentamicin again. Method 1. Bactericidal curve: Bacteria inoculated in logarithmic growth phase 5 × 10 ~ 7cfu / ml to BHI broth test in vitro bactericidal rate. The concentrations of antibiotics in the sera of various dosage regimens were selected to be 2 and 40 μg / ml for penicillins, 10 μg / ml for vancomycin and 4 μg / ml for gentamicin. Samples were serially diluted at 0, 3, 6 and 24 hours