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目的:研究聚乙二醇干扰素抗病毒治疗对慢性乙型肝炎(CHB)患者Th17、Treg及Th17/Treg的影响,及Th17/Treg与表面抗原的关系。方法:30例HBe Ag阴性慢性乙型肝炎患者予聚乙二醇干扰素治疗,在治疗前与治疗后24周、48周时,检测外周血Th17、Treg的细胞频数及表面抗原定量,并与20例健康人的Th17、Treg的细胞频数进行比较,分析聚乙二醇干扰素对Th17、Treg的影响。结果:1CHB患者的Th17及Treg细胞频数较健康人的高,但Th17/Treg比例较健康人低。抗病毒治疗后24周时,Th17/Treg比例较治疗前升高,48周时Th17/Treg比例较24周时稍降低(P>0.05),48周时与治疗前相比仍升高(P<0.05)。2治疗结束时表面抗原阴转病人的Th17/Treg比未阴转病人高。3慢乙肝病人的Th17/Treg与表面抗原定量成负相关(r=-0.388,P<0.05)。结论:CHB患者体内存在着Th17/Treg的失衡,干扰素可以调节CHB患者的免疫功能,有望实现表面抗原阴转。
Objective: To investigate the effect of pegylated interferon on Th17, Treg and Th17 / Treg in patients with chronic hepatitis B (CHB) and the relationship between Th17 / Treg and surface antigens. Methods: Thirty patients with HBeAg-negative chronic hepatitis B were treated with pegylated interferon. Before treatment and at 24 weeks and 48 weeks after treatment, the cell numbers and surface antigens of Th17 and Treg in peripheral blood were measured, The frequency of Th17 and Treg in 20 healthy volunteers was compared to analyze the effects of pegylated interferon on Th17 and Treg. Results: The frequency of Th17 and Treg cells in 1CHB patients was higher than that in healthy people, but the proportion of Th17 / Treg was lower than that in healthy people. The ratio of Th17 / Treg increased at 24 weeks after anti-virus treatment compared with that before treatment, and the ratio of Th17 / Treg slightly decreased at 48 weeks (P> 0.05) and increased at 48 weeks <0.05). 2 The Th17 / Treg of patients with negative surface antigen at the end of treatment is higher than those of patients without negative conversion. Th17 / Treg in chronic hepatitis B patients is negatively correlated with the quantification of surface antigens (r = -0.388, P <0.05). Conclusion: There is an imbalance of Th17 / Treg in CHB patients. Interferon can regulate the immune function of patients with CHB, and is expected to achieve negative conversion of surface antigens.