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目的从细胞水平探讨生长因子：肿瘤坏死因子－α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ，ＴＮＦ－α）、白细胞介素－１β（ｉｎｔｅｒｌｅｕｋｉｎ－１β，ＩＬ－１β）和碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）对视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞增殖的协同调控作用。方法通过ＲＰＥ细胞培养，采用氚标胸腺嘧啶核苷（３Ｈ－ｔｈｙｍｉｄｉｎｅ，３Ｈ－ＴｄＲ）掺入测定三种生长因子单用和分别联用诱导ＲＰＥ细胞ＤＮＡ合成改变，细胞计数观察ＲＰＥ细胞生长变化。结果三种生长因子单用均可明显促进ＲＰＥ细胞ＤＮＡ合成及细胞数目增加，ＴＮＦ－α、ＩＬ－１β和ｂＦＧＦ的３Ｈ－ＴｄＲ掺入每分钟计数值（ｃｏｕｎｔｓｐｅｒｍｉｎｕｔｅ，ｃｐｍ）分别是对照组的２．７４、２．６６和１．６９倍（Ｐ＜０．０５）。两种生长因子联用较单用ｃｐｍ明显提高，其中ＴＮＦ－α＋ＩＬ－１βｃｐｍ是对照组的３．１４倍（Ｐ＜０．０５）。三种生长因子联用时ｃｐｍ是对照组的３．７４倍（Ｐ＜０．０５）。结论三种生长因子间存在促ＲＰＥ细胞增殖的协同作用，这可能是生长因子对ＲＰＥ细胞增殖的?
OBJECTIVE: To investigate the effect of growth factor (TNF-α), interleukin-1β (IL-1β) and basic fibroblast growth factor (bFGF) Collective regulation of retinal pigment epithelium (RPE) cell proliferation. Methods RPE cells were cultured and labeled with 3H-thymidine (3H-TdR). The growth of RPE cells was observed by cell counting. Results The results showed that DNA synthesis and number of cells in RPE cells were significantly increased by all three growth factors. The 3H-TdR incorporation counts (cpm) of TNF-α, IL-1β and bFGF were 2 .74, 2.66 and 1.69 times (P <0.05) respectively. The combination of two kinds of growth factors significantly increased compared with the single use of cpm, which TNF-α + IL-1βcpm was 3.14 times that of the control group (P <0.05). The cpm of three growth factors combined was 3.74 times that of the control group (P <0.05). Conclusion The synergistic effect of the three growth factors on the proliferation of RPE cells may be due to the proliferation of RPE cells.