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目的:制备壳聚糖苦丁茶纳米粒并考察其理化性质和体外释药性能。方法:采用梯度体积分数乙醇提取苦丁茶总黄酮,利用紫外-分光光度法测定总黄酮含量,检测波长510nm。通过离子交联法制备壳聚糖苦丁茶纳米粒,考察其形态、粒子分布、包封率、载药量及体外释放情况。结果:苦丁茶中总黄酮质量分数31.84%。纳米粒呈球形,粒径200~400nm,多分散系数0.137。壳聚糖苦丁茶纳米粒最佳处方为壳聚糖1g·L-1,三聚磷酸钠1g·L-1,苦丁茶总黄酮1g·L-1。体外释放试验显示该纳米粒在前2h有突释效应,释放量45.6%~48.9%,24h总释放量91.9%。结论:壳聚糖纳米粒可作为苦丁茶的优良载体,为新型降脂制剂的开发提供参考。
OBJECTIVE: To prepare chitosan Kudingcha nanoparticles and investigate its physicochemical properties and in vitro drug release properties. Methods: The total flavonoids of Ku Ding Cha were extracted by gradient volume fractional ethanol, and the content of total flavonoids was determined by ultraviolet spectrophotometry. The detection wavelength was 510nm. Chitosan Kudingcha nanoparticles were prepared by ion-crosslinking method. The morphology, particle distribution, entrapment efficiency, drug loading and in vitro release were investigated. Results: The content of total flavonoids in Kuding tea was 31.84%. Nanoparticles spherical, particle size 200 ~ 400nm, polydispersity 0.137. Chitosan Kudingcha nanoparticles best prescription for chitosan 1g · L-1, sodium tripolyphosphate 1g · L-1, total kudingcha flavonoids 1g · L-1. In vitro release test showed that the nanoparticles had a burst effect in the first 2h, the release of 45.6% ~ 48.9%, 24h total release of 91.9%. Conclusion: Chitosan nanoparticles can be used as an excellent carrier of Kuding tea, and provide a reference for the development of new lipid-lowering agents.