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叶片是植物光合作用的主要场所,优良的叶片形态有利于塑造理想的株型,提高光合效率。为了研究叶片形态建成的分子机制,自水稻T-DNA插入突变体库中筛选获得1个叶片半卷曲的卷叶突变体(roll leaf mutant,命名为rlm1),突变体rlm1主要特征为成熟叶片沿中脉向内卷,叶片最终卷成直立半圆筒状,叶片卷曲度达0.64,叶片直立参数达95,且光合效率显著优于野生型。通过图位克隆技术,确定突变体rlm1突变位点位于LOC_Os03g06654基因的第1个内含子,LOC_Os03g06654基因编码黄素单氧化酶(flavin-containing monooxygenase),RT-PCR表达分析表明LOC_Os03g06654基因因T-DNA插入而导致完全失活。该基因与已报道的水稻卷叶基因Os COW1(Constitutively Wilted 1)为等位基因,而且突变体rlm1所表现的农艺性状均佳,可期待利用该突变体进行高光合的育种实践。
Leaf is the main site of photosynthesis of plants, and good leaf shape is conducive to shaping the ideal plant type, improve photosynthetic efficiency. In order to study the molecular mechanism of leaf morphogenesis, one leaf semi-curly leaf roll mutant (named rlm1) was screened from the rice T-DNA insertion mutant library. The main characteristics of the mutant rlm1 were mature leaf edge The midrib was inwardly rolled, and the leaves were eventually rolled into an upright semi-cylindrical shape with leaf curl of 0.64 and leaf upright parameters of 95, with significantly better photosynthetic efficiency than the wild type. The first intron of LOC_Os03g06654 gene was identified by map-based cloning technique. The LOC_Os03g06654 gene encoded flavin-containing monooxygenase. RT-PCR analysis showed that the LOC_Os03g06654 gene was inserted into T_DNA Which led to complete inactivation. This gene is closely related to the reported gene of rice curly leaf Os COW1 (Constitutively Wilted 1), and the agronomic trait exhibited by the mutant rlm1 is excellent. Therefore, it is expected that the mutant will be used for breeding high photosynthesis.