论文部分内容阅读
目的观察体外培养的人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUCMSCs)对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)的治疗作用。方法组织块贴壁法培养HUCMSCs、流式细胞术鉴定。用视网膜光感受器间维生素A类结合蛋白(interphotoreceptor retinoid-binding protein,IRBP)诱导EAU模型。C57BL/6小鼠尾静脉注射HUCMSCs,每日用裂隙灯显微镜对小鼠眼前节的炎症情况进行观察。流式细胞术检测实验小鼠脾脏淋巴细胞CD11c、CD86表型。结果建模后的C57BL/6小鼠于注射IRBP后7~9 d开始发病,12~14 d发病达高峰,16~20 d眼前节炎症自限性减轻,21 d后眼内炎症逐渐消退、自愈。HUCMSCs治疗后,建模小鼠EAU发病时间延长,炎症程度减轻,实验组与模型对照组比较,差异有统计学意义(P<0.05)。建模后的小鼠脾脏CD11c+CD86-细胞数量增加非常明显,经HUCMSCs治疗后第9天CD11c~+CD86~-细胞数量接近治疗前水平。建模后小鼠脾脏中CD86+CD11c+/CD11c比值下降,经HUCMSCs治疗后有所回升,但在第15天时仍未恢复至正常水平。结论 HUCMSCs对IRBP诱导的小鼠EAU有治疗作用,HUCMSCs作用于树突状细胞等抗原呈递细胞,抑制了EAU的进展。
Objective To observe the therapeutic effect of human umbilical cord mesenchymal stem cells (HUCMSCs) cultured in vitro on experimental autoimmune uveitis (EAU). Methods HUCMSCs were cultured with adherent method and identified by flow cytometry. The EAU model was induced by retinal photoreceptor inter-retinal-binding protein (IRBP). C57BL / 6 mice were injected intravenously with HUCMSCs, and the inflammation of mice anterior segment was observed daily with slit lamp microscope. Flow cytometry was used to detect the CD11c and CD86 phenotypes of spleen lymphocytes in experimental mice. Results The C57BL / 6 mice began to develop at 7-9 days after injection of IRBP. The incidence peaked at 12-14 days. The self-limiting inflammation of the anterior segment of the eye reduced after 16-20 days. The inflammation in the eye subsided gradually after 21 days, Self-healing. After HUCMSCs treatment, the onset time of EAU in model mice was prolonged and the degree of inflammation was alleviated. The difference between the experimental group and the model control group was statistically significant (P <0.05). The number of CD11c + CD86- cells in the spleens of mice increased significantly after modeling. The number of CD11c ~ + CD86 ~ - cells approached the pre-treatment level on the 9th day after HUCMSCs treatment. After modeling, the ratio of CD86 + CD11c + / CD11c in the spleen of mice decreased after treatment with HUCMSCs, but did not recover to the normal level on the 15th day. Conclusion HUCMSCs can induce IRBP-induced EAU in mice. HUCMSCs act on antigen-presenting cells such as dendritic cells and inhibit the progression of EAU.