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目的:观察N-甲基-D-天冬氨酸受体亚单位1(N-Methyl-D-aspartate receptor subunit1,NR1)在幼年不同时期大鼠脑室下区(subventricular zone,SVZ)相同部位以及生后14d(表达高峰期)大鼠侧脑室不同部位旁的SVZ表达状况,为研究NR1是否在神经发生中起作用提供组织学依据。方法:生后7,14,21,28d的SD大鼠脑6μm厚石蜡切片,免疫组化ABC法显色,图像分析及统计处理。结果:NR1在生后7,14,21,28d大鼠SVZ均有表达,14d时表达至高峰;NR1在14d大鼠侧脑室不同部位旁SVZ内也均有表达,且侧脑室外侧角旁及下角旁SVZ表达最高。NR1阳性细胞有多种形态,均以胞膜着色为主,胞浆浅淡,偶可见核仁。结论:NR1在幼年不同时期大鼠SVZ及14d大鼠侧脑室不同区域旁SVZ均有表达,但表达高低因时间及部位而异,提示NR1在幼年大鼠SVZ的表达具有时空特异性,并可能在神经发生过程中发挥作用。
Objective: To observe the effect of N-Methyl-D-aspartate receptor subunit 1 (NR1) on the same site of subventricular zone (SVZ) The expression of SVZ next to different parts of the lateral ventricle in rats at 14 days after birth was used to provide a histological basis for studying whether NR1 plays a role in neurogenesis. Methods: Sprague - Dawley rats were exposed to 6 μm thick paraffin sections at 7, 14, 21 and 28 days after birth. The cells were stained with immunohistochemical ABC method and analyzed by image analysis. Results: NR1 was expressed in SVZ of rats at 7, 14, 21 and 28 days after birth, and peaked at 14 days. NR1 was also expressed in SVZ adjacent to different parts of lateral ventricle in 14 days, SVZ expression next to the highest. NR1 positive cells have a variety of forms, are dominated by membrane staining, pale cytoplasm, and even visible nucleoli. CONCLUSIONS: The expression of NR1 in SVZ of SVZ rats and SVS in different regions of lateral ventricle of rats at different stages of childhood is different, but the expression level varies with time and location. It suggests that NR1 may have spatiotemporal specificity in SVZ expression in young rats and may be In the process of neurogenesis play a role.