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从感染的沙鼠腹腔收集彭亨和马来丝虫,分别从犬末稍血及心脏获取犬恶丝虫微丝蚴和成虫,棉鼠丝虫取自感染棉鼠的胸腔。所有成虫用盐水洗涤数次,雌、雄虫分开。从沙鼠腹腔收集的微丝蚴,用PBS与等量的1%琼脂糖混合液冲洗,按Tomisato法将从琼脂糖凝胶中逸出的微丝蚴置入Hanks液以纯化微丝蚴。成虫混悬于碳酸盐缓冲液(0.05M,pH9.5),超声捣碎,搅动24小时,
Paenibacillus and Malayi were collected from the abdominal cavity of infected gerbils, and filarial and microflora of filarias were obtained respectively from the blood and heart at the end of the dog. The silkworm was taken from the chest of infected cotton rats. All adults washed with saline several times, female and male separated. Filariae collected from the peritoneal cavity of a gerbil were washed with PBS and an equal amount of 1% agarose, and the microfilariae escaping from the agarose gel were put into a Hanks’ solution according to the Tomisato method to purify the microfilariae. Adults were suspended in carbonate buffer (0.05 M, pH 9.5), sonicated, agitated for 24 hours,