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目的研究红景天苷(salidroside,SDS)对百草枯(paraquat,PQ)中毒大鼠肺组织TGF-β1表达的影响。方法将130只SD大鼠随机分为正常对照组、模型对照组、SDS治疗组,后2组又分为1,3,7,14,21,28 d 6个亚组,每亚组10只。模型对照组和SDS治疗组采用一次性灌胃百草枯溶液(20 mg·m L?1)制备急性PQ中毒肺损伤模型,染毒1 h后分别给予相同体积的生理盐水、SDS(10 mg·m L?1)腹腔注射。各组在相应时间点取材,采用免疫组化、RT-PCR测定肺组织中转化生长因子β1(TGF-β1)及其m RNA的表达情况。结果免疫组化结果显示,与正常对照组比较,模型对照组、SDS治疗组肺组织TGF-β1表达显著增加(P<0.05),在第14天达到最大值。与模型对照组各时间点比较,SDS治疗组中TGF-β1的表达量低,两者差异有统计学意义(P<0.05)。RT-PCR结果显示模型组与SDS治疗组染毒后第1天大鼠肺组织TGF-β1m RNA水平开始增加,在第14天达到最高值,第28天基因表达恢复正常。第3天至第28天SDS治疗组TGF-β1 m RNA表达量比模型对照组低,两者比较差异有统计学意义(P<0.05)。结论 TGF-β1参与了PQ中毒大鼠肺纤维化病理生理过程,SDS能抑制TGF-β1的表达,减轻PQ中毒大鼠肺损伤。
Objective To investigate the effect of salidroside (SDS) on the expression of TGF-β1 in the lung tissue of paraquat (PQ) -owned rats. Methods 130 SD rats were randomly divided into normal control group, model control group and SDS treatment group. The latter 2 groups were divided into 6 subgroups at 1,3,7,14,21,28 d, 10 subgroups . The model control group and the SDS treatment group were given a single dose of paraquat solution (20 mg · mL -1) to prepare the model of acute lung injury induced by PQ. After being treated for 1 h, the rats were given the same volume of normal saline, SDS (10 mg · mL -1) m L? 1) intraperitoneal injection. The rats in each group were drawn at corresponding time points, and the expression of transforming growth factor-β1 (TGF-β1) and its m RNA in lung tissue was detected by immunohistochemistry and RT-PCR. Results The results of immunohistochemistry showed that compared with the normal control group, the expression of TGF-β1 in model control group and SDS treatment group was significantly increased (P <0.05), reaching the maximum on the 14th day. Compared with the model control group at each time point, the expression of TGF-β1 in SDS-treated group was low, the difference was statistically significant (P <0.05). RT-PCR results showed that the level of TGF-β1mRNA in the lung tissue of model group and SDS treatment group began to increase on the first day after exposure, reaching the highest value on the 14th day, and the gene expression returned to normal on the 28th day. From the third to the 28th day, the expression of TGF-β1 m RNA in the SDS-treated group was lower than that in the model control group, and the difference was statistically significant (P <0.05). Conclusion TGF-β1 is involved in the pathophysiological process of pulmonary fibrosis in rats with PQ poisoning. SDS can inhibit the expression of TGF-β1 and alleviate lung injury in PQ-poisoned rats.