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目的 研究放射损伤小鼠骨髓基质细胞对粒系祖细胞生长的影响。方法 采用体外小鼠骨髓基质细胞贴壁培养法和粒系祖细胞 (CFU GM)培养法。结果 伤后 3～ 7d ,有基质细胞贴壁的CFU GM生长明显低于无贴壁组 ;伤后 14d贴壁层形成后 ,放射损伤组的CFU GM生长明显低于正常对照组 ;放射损伤后 3～ 7d ,骨髓基质细胞可抑制正常CFU GM生长 ,用薄层琼脂分隔基质细胞贴壁层与靶细胞 ,其抑制作用减弱。当无外源性集落刺激因子 (CSF)时 ,基质细胞贴壁层对CFU GM生长仍显示一定刺激作用。结论 辐射使骨髓基质细胞功能受到一定损伤 ,但在放射损伤造血修复与重建过程中 ,骨髓基质细胞仍起着“刺激”与“抑制”双重的动态平衡调控作用
Objective To investigate the effect of bone marrow stromal cells (BMMNC) on the growth of myeloid progenitor cells (BMMNCs) in irradiated mice. Methods Mouse bone marrow stromal cells adherent culture method and myeloid progenitor cells (CFU GM) culture method were used. Results The CFU GM growth of stromal cells adhered to the basilar membrane was significantly lower than that of the non-adherent group 3 to 7 days after injury. After the adherent layer was formed 14 days after injury, the CFU GM growth of the radiation injury group was significantly lower than that of the normal control group 3 ~ 7d, bone marrow stromal cells can inhibit the growth of normal CFU GM thin layer of agar separation stromal cells and target cells, its inhibition weakened. In the absence of exogenous colony stimulating factor (CSF), stromal cell adherent layer of CFU GM growth still showed some stimulation. Conclusion Radiation can impair the function of bone marrow stromal cells. However, in the course of hematopoietic repair and reconstruction after radiation injury, bone marrow stromal cells still play a dual role of “stimulus” and “suppression”