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[Objective]To observe the effects of extracts from RADIX CURCUMAE grown in Guangxi on the human hepatic stellate cells,and to find out its effects on proliferation and apoptosis rate. [Methods] After drug incubation of human hepatic stellate cells( HSC-LX2) for48 h,inhibition rate of cell proliferation was detected by MTT( methyl thiazolyl tetrazolium) colorimetric method; cell apoptosis in each status was measured by flow cytometry( ANNEXIN V-PE /7-AAD double staining).[Results] Extracts in low-,middle- and high-dosage groups of RADIX CURCUMAE could restrict the proliferation of HSC-LX2. The inhibitory rates at 48 h were 15. 4%,49. 1% and 58. 3%,respectively.IC50 value at 48 h was 45 μmol / L. Early and late apoptosis of cells could be induced. The percentages of total apoptotic cells at 48 h were20. 7%,29. 8% and 34. 4%,respectively.[Conclusions] Extracts from RADIX CURCUMAE grown in Guangxi could restrict the HSC-LX2 proliferation and induce the cell apoptosis,so as to affect the hepatic fibrosis. All these abilities mentioned above showed a dose-dependent manner. And these abilities in middle- and high-dosage groups of RADIX CURCUMAE were significantly stronger than those in colchicine group and compound Biejiaruangan Tablet group.
[Objective] To observe the effects of extracts from RADIX CURCUMAE grown in Guangxi on the human hepatic stellate cells, and to find out its effects on proliferation and apoptosis rate. [Methods] After drug incubation of human hepatic stellate cells (HSC-LX2) for48 h, inhibition rate of cell proliferation was detected by MTT (methyl thiazolyl tetrazolium) colorimetric method; cell apoptosis in each status was measured by flow cytometry (ANNEXIN V-PE / 7-AAD double staining). [Results] , middle- and high-dosage groups of RADIX CURCUMAE could restrict the proliferation of HSC-LX2. The inhibitory rates at 48 h were 15. 4%, 49.1% and 58.3%, respectively. IC50 value at 48 h was 45%> respectively, early and late apoptosis of cells could be induced. The percentages of total apoptotic cells at 48 h were 20.7%, 29.8% and 34.4%, respectively. [Conclusions] Extracts from RADIX CURCUMAE grown in Guangxi could restrict the HSC-LX2 proliferation and induce the cell apoptosis, so as to affect the h And these abilities in middle- and high-dosage groups of RADIX CURCUMAE were significantly stronger than those in colchicine group and compound Biejiaruangan Tablet group.