一、母种、原种、栽培种的制作过程(一)母种:母种也就是一级菌种,它的制作大致经过以下几个过程:培养基配制→培养基灭菌→子实体分离或菇木分离或孢子分离→培养→转管纯化培养→母种。1.母种培养基配制。应根据不同食用菌的不同营养需要配制不同的培养基。常用的配方有:配方①:土豆葡萄糖琼脂培养基(简称PDA培养基) First, the mother species, original species, cultivation of the production process (A) Mother: Mother is also a strain, its production through the following process: Preparation of medium → medium sterilization → fruiting body separation Or mushroom separation or spore separation → culture → tube purification culture → mother species. 1. Mother culture medium preparation. Should be based on the different nutritional needs of different mushroom preparation of different media. Commonly used formula are: Recipe ①: potato dextrose agar (referred to as PDA medium)