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目的:观察CK7、CK15、CK20、CD10、Bcl-2、Nestin在基底细胞癌(basal cell carcinoma,BCC)和毛母细胞瘤(trichoblastoma,TB)中的表达,探讨其在两种肿瘤诊断和鉴别诊断中的作用。方法:对确诊的35例BCC和7例TB石蜡包埋标本行CK7、CK15、CK20、CD10、Bcl-2、Nestin免疫组化染色,观察它们在肿瘤细胞及其周围间质中的表达。结果:对各种抗体在基底细胞癌与毛母细胞瘤表达分布比较,CK7、CK15、CK20、Nestin表达分布无明显差异。Bcl-2在基底细胞癌中仅癌巢周边细胞阳性,间质部分细胞灶状阳性,而毛母细胞瘤中上皮细胞均弥漫弱阳性。CD10在两种肿瘤中周边细胞均阳性表达,但基底细胞癌中间质阴性,而毛母细胞瘤中间质阳性表达。结论:分析CD10和Bcl-2在肿瘤中表达分布的不同,有助于这两种肿瘤的鉴别诊断。
Objective: To investigate the expression of CK7, CK15, CK20, CD10, Bcl-2 and Nestin in basal cell carcinoma (BCC) and trichoblastoma (TB) The role of diagnosis. Methods: The expression of CK7, CK15, CK20, CD10, Bcl-2 and Nestin in paraffin embedded specimens of 35 cases of BCC and 7 cases of TB confirmed by immunohistochemical staining were observed in tumor cells and their surrounding stroma. Results: There was no significant difference in the distribution of CK7, CK15, CK20 and Nestin between basal cell carcinoma and hairy cell tumor. In basal cell carcinoma, Bcl-2 only possesses positive cells in the periphery of cancer neoplasms and positive cells in interstitial part, while epithelial cells in the hair follicle cells are diffusely weakly positive. CD10 expression was positive in peripheral cells in both tumors, but stroma was negative in basal cell carcinoma while stroma was expressed in meiblastoma. Conclusion: It is helpful to differentiate the expression of CD10 and Bcl-2 in the differential diagnosis between the two tumors.