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目的:研究耐力胶囊对辐射损伤小鼠的保护作用,为开发有效的抗辐射药物提供实验依据。方法:雄性昆明种小鼠,分为辐射模型组、阳性药芪胶升白胶囊组(0.98 g·kg-1)及耐力胶囊低、中、高3个剂量(分别为1.82,3.64,7.28 g·kg-1)组,连续ig 7 d后,实验分两部分:经8.5 Gy60Coγ射线照射,观察耐力胶囊对小鼠30 d存活率,平均生存时间的影响;6.5 Gy60Coγ射线照射,观察耐力胶囊对辐射损伤小鼠的外周血细胞、骨髓有核细胞计数、免疫器官指数、骨髓组织病理的影响。结果:耐力胶囊中剂量组可以提高受照小鼠30 d存活率(提高存活率为37.5%),延长受照小鼠平均存活时间(24.88 d),保护系数达1.30;可以升高受照小鼠的外周血白细胞(辐射后3,7 d,WBC计数分别为1.34×109/L,0.89×109/L;),增加辐射后7 d小鼠免疫器官指数(胸腺和脾脏指数分别为0.68,0.98 mg·g-1)和骨髓有核细胞计数(5.89×106/股骨),减轻小鼠骨髓造血细胞的损伤。结论:耐力胶囊对60Coγ射线损伤小鼠具有保护作用。
Objective: To study the protective effect of Nai Li Capsule on radiation-injured mice and provide experimental basis for the development of effective anti-radiation drugs. Methods: Male Kunming mice were divided into three groups: radiation model group, positive XGS capsule group (0.98 g · kg-1), and low, medium and high doses of Endurance Capsule (1.82, 3.64 and 7.28 g · Kg-1) group. After continuous ig for 7 days, the experiment was divided into two parts: The effect of Endurance Capsule on 30-day survival rate and mean survival time of mice was observed by 8.5 Gy60Coγ ray irradiation; 6.5 Gy60Coγ ray irradiation was used to observe the effect of Endurance Capsule Effects of radiation-injured mice on peripheral blood cells, bone marrow nucleated cells count, immune organ index and bone marrow histopathology. Results: The dose-tolerant capsule group could increase the 30-day survival rate (37.5% survival rate), prolong the average survival time (24.88 days) and the protection coefficient of 1.30 in irradiated mice; (WBC count of 1.34 × 109 / L, 0.89 × 109 / L, respectively) on day 7 and day 7 after irradiation, and increased the index of immune organ of thymus and spleen of mice 7 days after radiation (the index of thymus and spleen were 0.68, 0.98 mg · g-1) and bone marrow nucleated cells count (5.89 × 106 / femur) to reduce the damage of mouse bone marrow hematopoietic cells. Conclusion: Endurance Capsule has protective effects on 60Co γ-ray-injured mice.